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2.3.1.211: bisdemethoxycurcumin synthase

This is an abbreviated version!
For detailed information about bisdemethoxycurcumin synthase, go to the full flat file.

Word Map on EC 2.3.1.211

Reaction

2 4-coumaroyl-CoA +

malonyl-CoA
+
H2O
= 3 CoA +
bisdemethoxycurcumin
+ 2 CO2

Synonyms

ClPKS10, curcumin synthase, curcuminoid synthase, CUS, Os07g0271500, OsCUS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.211 bisdemethoxycurcumin synthase

Cloned

Cloned on EC 2.3.1.211 - bisdemethoxycurcumin synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ClPKS10, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, tissue expression analysis and quantification by real-time PCR
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene AK109558, expression of His6-tagged CUS in Escherichia coli strain BL21(DE3)-pLysS
-
PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS genes, are introduced into Escherichia coli