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2.3.1.184: acyl-homoserine-lactone synthase

This is an abbreviated version!
For detailed information about acyl-homoserine-lactone synthase, go to the full flat file.

Word Map on EC 2.3.1.184

Reaction

an acyl-[acyl-carrier protein]
+
S-adenosyl-L-methionine
=
an [acyl-carrier protein]
+
S-methyl-5'-thioadenosine
+
an N-acyl-L-homoserine lactone

Synonyms

3-oxo-C12-HSL synthase, AbaI, ACII, acyl homoserine lactone synthase, acyl-homoserine lactone synthase, acyl-homoserine-lactone synthase, acyl-homoserinelactone synthase, acyl-HSL synthase, acylhomoserine lactone synthase, AHL synthase, AHS, AHSL synthase, AhyI, AinS, AinS protein, AurI, autoinducer synthase, autoinducer synthesis protein rhlI, C4-HSL synthase, C6-AHL synthase, CsaI, EsaI, ExpI, expIEcz, ExpISCC1, ExpISCC3065, hdtS, HHL synthase, LasI, LasI synthase, LasI/R, LasR, LuxI, LuxI protein, LuxM, More, MrlI1, MrlI2, MrtI, mtqI, N-acyl homoserine lactone synthase, N-acyl-homoserine lactone synthase, N-hexanoyl homoserine lactone synthase, NmuI, Nmul_A2390, PgaI, PhzI, PppuI, PsyI, RhlI, RhlI/R, signal synthase CviI, SmaI, SpnI, TofI, TraI, YenI, YpeRI, YspI, YspRI

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.184 acyl-homoserine-lactone synthase

Purification

Purification on EC 2.3.1.184 - acyl-homoserine-lactone synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recobinant maltose binding protein-fusion AinS from Escherichia coli by amylose affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
-
recombinant His-tagged LasIDELTAG from Escherichia coli by nickel affinity chromatography and gel filtration, the His-tag is removed by thrombin cleavage
-
recombinant maltose binding protein-fusion enzyme RhlI from Escherichia coli strain XL 1-Blue by amylose affinity chromatography, recombinant His-tagged RhlI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant maltose-binding protein-fusion enzyme from Escherichia coli strain XL 1-Blue by amylose affinity chromatography
-
recombinant RhlI 95fold from Escherichia coli strain XL 1-Blue by anion and cation exchange chromatography and gel filtration
-