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2.3.1.184: acyl-homoserine-lactone synthase

This is an abbreviated version!
For detailed information about acyl-homoserine-lactone synthase, go to the full flat file.

Word Map on EC 2.3.1.184

Reaction

an acyl-[acyl-carrier protein]
+
S-adenosyl-L-methionine
=
an [acyl-carrier protein]
 +
S-methyl-5'-thioadenosine
 +
an N-acyl-L-homoserine lactone

Synonyms

3-oxo-C12-HSL synthase, AbaI, ACII, acyl homoserine lactone synthase, acyl-homoserine lactone synthase, acyl-homoserine-lactone synthase, acyl-homoserinelactone synthase, acyl-HSL synthase, acylhomoserine lactone synthase, AHL synthase, AHS, AHSL synthase, AhyI, AinS, AinS protein, AurI, autoinducer synthase, autoinducer synthesis protein rhlI, C4-HSL synthase, C6-AHL synthase, CsaI, EsaI, ExpI, expIEcz, ExpISCC1, ExpISCC3065, hdtS, HHL synthase, LasI, LasI synthase, LasI/R, LasR, LuxI, LuxI protein, LuxM, More, MrlI1, MrlI2, MrtI, mtqI, N-acyl homoserine lactone synthase, N-acyl-homoserine lactone synthase, N-hexanoyl homoserine lactone synthase, NmuI, Nmul_A2390, PgaI, PhzI, PppuI, PsyI, RhlI, RhlI/R, signal synthase CviI, SmaI, SpnI, TofI, TraI, YenI, YpeRI, YspI, YspRI

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.184 acyl-homoserine-lactone synthase

Cloned

Cloned on EC 2.3.1.184 - acyl-homoserine-lactone synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expression in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli
functional overexpression of His-tagged LasIDELTAG and point mutants in Escherichia coli as soluble proteins
-
gene abaI, DNA and amino acid sequence determination and analysis in the different clinical isolates
-
gene ainS, expression of AinS as maltose binding protein-fusion enzyme in Escherichia coli strain XL 1-Blue
-
gene cviI, a luxI homologue, DNA and amino acid sequence determination and analysis, expression in the wild-type strain KT2440 and a ppoR (luxR homologue) mutant of Pseudomonas putida, the luxI homologue is located on a broad-host-range plasmid. The recombinant PpoR-HHL complex is not involved in the response to oxidative stresses caused by paraquat, hydrogen peroxide, cumene hydroperoxide, and salicylate, but responds to antibiotic stress caused by ampicillin, strain KT2440 harbouring the pHHL plasmid evidences significantly reduced attachment to a polystyrene surface, phenotype, detailed overview. Identification of 71 HHL-signalling target genes regulated by PpoR in the recombinant strain
-
gene expI, is transcribed convergently and overlapping at their 3' ends with gene expR
-
gene expISCC1, expression of wild-type and mutant enzymes in Escherichai coli, inoculation of Solanum tuberosum tubers
gene lasI, genetic organization and las genetic locus
-
gene luxI, expression as maltose-binding protein-fusion enzyme in Escherichia coli strain XL 1-Blue
-
gene ppuI, genetic organization and ppu genetic locus
-
gene rhlI, expression in Escherichia coli strain XL 1-Blue
-
gene rhlI, overexpression in Escherichia coli strain XL 1-Blue as maltose binding protein-fusion protein, overexpression of His-tagged RhlI in Escherichia coli strain BL21(DE3)
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gene yspI, expression of wild-type enzyme in Escherichia coli strain BL21(DE3), the recombinant enzyme shows a similar substrate profile as the native enzyme
-
genes esaI and lasI, expression of wild-type and mutant enzymes in Escherichia coli
-
heterologous overexpression in Escherichia coli
overexpression in Escherichia coli Rosetta (DE3)
overexpression of His-tagged enzyme in Escherichia coli straoin BL21(DE3)
-
recombinant maltose binding protein-fusion protein AhyR binds to the ahyI promoter region
-
the abaI gene is cloned into the pBluescript SK vector