2.3.1.183: phosphinothricin acetyltransferase

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For detailed information about phosphinothricin acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.183

2.3.1.183

pat

herbicide

glufosinate

bialaphos

viridochromogenes

herbicide-resistant

basta

hygroscopicus

aryloxyalkanoate

herbicide-tolerant

nopaline

microprojectile

biotechnology

nptii

glufosinate-resistant

agriculture

analysis

glufosinate-ammonium

non-gm

bialaphos-resistant

regenerable

molecular biology

Reaction

Synonyms

BAR, DR_1182, GK0593, GK2920, hpat, L-PPT N-acetyltransferase, MAT, methionine sulfone N-acetyltransferase, Pat, phosphinothricin acetyltransferase, phosphinothricin N-acetyltransferase, phosphinothricin-N-acetyltransferase, PitA, PPT acetyltransferase

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.183 phosphinothricin acetyltransferase

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Results	in table
1	Activating Compound
2014	AA Sequence
22	Application
1	CAS Registry Number
26	Cloned(Commentary)
11	Cofactor
2	Crystallization (Commentary)
6	General Information
1	General Stability
1	Inhibitors
8	kcat/KM [mM/s]
17	KM Value [mM]
10	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
65	Organism
4	Pathway
8	PDB ID
9	pH Optimum
3	pH Range
8	Protein Variants
12	Purification (Commentary)
1	Reaction
5	Reaction Type
49	Reference
11	Source Tissue
8	Specific Activity [micromol/min/mg]
58	Substrates and Products (Substrate)
6	Subunits
59	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Temperature Stability [°C]
12	Turnover Number [1/s]

pH Range

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pH Range on EC 2.3.1.183 - phosphinothricin acetyltransferase

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6 - 9

Streptomyces hygroscopicus

no activity at pH 5.0 and pH 10.0

673824

7 - 9.5

Nocardia sp. AB2253

B0I560

Catalytic activity reaches a maximum at pH 8 to pH 8.5. In Tris buffer at pH 7 and pH 9.5 20-30% catalytic activity remains. In phosphate buffer, the activity at pH 7 and pH 6.5 is 15% and 10%, respectively. At pH 6 the enzyme shows no activity.