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2.3.1.183: phosphinothricin acetyltransferase

This is an abbreviated version!
For detailed information about phosphinothricin acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.183

Reaction

acetyl-CoA
+
Phosphinothricin
=
CoA
 +
N-acetylphosphinothricin

Synonyms

BAR, DR_1182, GK0593, GK2920, hpat, L-PPT N-acetyltransferase, MAT, methionine sulfone N-acetyltransferase, Pat, phosphinothricin acetyltransferase, phosphinothricin N-acetyltransferase, phosphinothricin-N-acetyltransferase, PitA, PPT acetyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.183 phosphinothricin acetyltransferase

Purification

Purification on EC 2.3.1.183 - phosphinothricin acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
for the purification of PAT from Pseudomonas fluorescens ammonium sulfate precipitation, a Source 15 Phenyl XK hydrophobic interaction column, and a Superdex 75 XK gel filtration column are used
-
recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, anion exchange, hydrophobic interaction, and hydroxylapatite chromatography, followed by gel filtration, to about 90% purity
-
recombinant enzyme from Pseudomonas fluorescens to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant enzyme from transgenic maize and rape plants by immunoaffinity chromatography, method development, overview
simple, time-saving, inexpensive and reproducible purification method employs a single chromatography step using a reactive dye resin, Reactive brown 10-agarose. Reactive brown 10 preferentially binds the phosphinothricin acetyltransferase protein, which can then be specifically released by acetyl-CoA. Phosphinothricin acetyltransferase protein can be purified to homogeneity from cottonseed with high recovery efficiency and enzymatically active
the recombinant protein is purified by Ni2+ affinity chromatography and used for the production of polyclonal antibodies, for Western blot analysis the protein is extracted from peppers
-