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2.3.1.180: beta-ketoacyl-[acyl-carrier-protein] synthase III

This is an abbreviated version!
For detailed information about beta-ketoacyl-[acyl-carrier-protein] synthase III, go to the full flat file.

Word Map on EC 2.3.1.180

Reaction

acetyl-CoA
+
a malonyl-[acyl-carrier protein]
=
an acetoacetyl-[acyl-carrier protein]
 +
CoA
 +
CO2

Synonyms

3-ketoacyl ACP synthase III, 3-ketoacyl acyl carrier protein synthase III, 3-ketoacyl carrier protein synthase III, 3-ketoacyl-(acyl carrier protein) synthase IIIA, 3-ketoacyl-(acyl carrier protein) synthase IIIB, 3-ketoacyl-ACP synthase III, 3-ketoacyl-acyl carrier protein synthase III, 3-ketoacyl-acyl-carrier protein synthase III, 3-oxoacyl-ACP synthase III, 3-oxoacyl-acyl carrier protein synthase III, 3-oxoacyl-[acyl-carrier-protein] synthase III, 3-oxoacyl:ACP synthase III, AbyA1, acetoacetyl-ACP synthase, acetyl-CoA:ACP transacylase, acetyl-CoA:malonyl-[acyl-carrier-protein] C-acyltransferase, ACO synthase III, ACP synthase III, AknE2, AlnI, AsuC3, AsuC4, AviN, BenQ, beta-ketoacyl (acyl carrier protein) synthase III, beta-ketoacyl acyl carrier protein synthase III, beta-ketoacyl acyl-carrier protein synthase III, beta-ketoacyl-(ACP) synthase III, beta-ketoacyl-(acyl-carrier-protein) synthase III, beta-ketoacyl-ACP III, beta-ketoacyl-ACP synthase, beta-ketoacyl-ACP synthase III, beta-ketoacyl-ACP synthase III-like protein, beta-ketoacyl-ACP-synthase III, beta-ketoacyl-acyl carrier protein (ACP) synthase III, beta-ketoacyl-acyl carrier protein synthase III, beta-ketoacyl-acyl carrier protein synthase-III, beta-ketoacyl-[acyl-carrier protein (ACP)] synthase III, beta-ketoacyl-[acyl-carrier-protein] synthase III, beta-ketoacyl:acyl carrier protein synthase III, beta-ketoacylacylcarrier protein synthase III, beta-ketobutyryl-ACP synthase, BomK, CalO4, CerJ, ChlB3, ChlB6, CorB, DarB, DpsC, ecFabH, ecKAS III, efFabH, EsmD1, EvrI, FabH, FabH1, FabH2, FabH3, FabHA, FabHB, fatty acid biosynthesis, enzyme H, fatty acid synthase type II condensing enzyme, FdmS, FrenI/FrnI, HedS, hiFabH, initiation ketosynthase, JcKAS III, KAS III, KAS III-like protein, KAS IIIA, KAS IIIB, KAS-III, KAS3a, KAS3b, KASIII, KijB, LstA, LstAB, LstB, mtFabH, MxnB, nmFabH, NphT7, NzsF, paFabH, PpyS, PqsBC, PqsD, PtmR, RedP, RevR, RkD, saFabH, SalQ/Orf12, short-chain condensing enzyme, spFabH, spyFabH, SsfN, TcsB, ThgI, TiaF, Tmn15, [acyl-carrier-protein] synthase III

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.180 beta-ketoacyl-[acyl-carrier-protein] synthase III

Purification

Purification on EC 2.3.1.180 - beta-ketoacyl-[acyl-carrier-protein] synthase III

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3500fold to homogeneity from leaves in an eight-step procedure including ACP affinity chromatography
-
immobilized metal ion affinity chromatography
immobilized metal ion affinity chromatography (Ni2+)
native enzyme 147fold from leaves by ammonium sulfate fractionation, ion exchange chromatography, hydrophobic interaction chromatography on a butyl resin, and affinity chromatography, recombinant GST-fusion enzyme from Escherichia coli by glutathione affinity chromatography, GST is cleaved off by thrombin
Ni-NTA column chromatography
Q-Sepharose, MonoQ, and hydroxyapatite column chromatographies (native enzyme) or Ni-NTA agarose column chromatography (selenomethionine-substituted enzyme)
recombinant enzyme from strain JT2038 by ammonium sulfate fractionation, gel filtration, and affinity chromatography
recombinant enzyme from Streptomyces lividans by ammonium sulfate fractionation, gel filtration, ion exchange and hydrophobic interaction chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and anion exchange chromatography, followed by ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged enzyme from Mycobacterium bovis strain BCG
-
recombinant His-tagged enzyme from strain BL21(DE3)
-
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography and gel filtration, the tag is cleaved off by thrombin
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3)
-
recombinant His-tagged wild-type and mutant enzymes from strain C41(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant FabHs from Mycobacterium bovis strain BCG by nickel affinity chromatography
recombinant His2-tagged enzyme from Escherichia coli by nickel and Blue Sepharose affinity chromatography, and gel filtration
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinty chromatography, tag cleavage by TEV protease, gel filtration, and ultrafiltration to over 90% purity
recombinant N-terminally His6-tagged FabH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant native and selenomethionine-labeled enzyme from Escherichia coli strain DH10B to homogeneity by two different steps of anion exchange and a step of hydroxyapatite chromatography
recombinant selenomethionine-labeled enzyme by ion exchange, hydroxylapatite, and affinity chromatography, and gel filtration
-
Sepharose column chromatography
-
to homogeneity in three chromatographic steps (Q-Sepharose, MonoQ, and hydroxyapatite)
-
to homogeneity in three chromatographic steps (Q-Sepharose, MonoQ, and hydroxyapatite), immobilized metal ion affinity chromatography (Ni2+)
-
truncated construct encoding residue 50 - 371