2.3.1.18: galactoside O-acetyltransferase

This is an abbreviated version!
For detailed information about galactoside O-acetyltransferase, go to the full flat file.

Reaction

Synonyms

acetyltransferase, galactoside, galactoside acetyltransferase, galactoside O-acetyltransferase, galactoside transacetylase, GAT, LacA, SACOL2570, thiogalactoside acetyltransferase, thiogalactoside transacetylase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.18 galactoside O-acetyltransferase

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Results	in table
902	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
1	Expression
2	General Information
11	Inhibitors
2	Ki Value [mM]
38	KM Value [mM]
3	Localization
3	Metals/Ions
8	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
27	Organism
12	PDB ID
2	pH Optimum
11	Protein Variants
9	Purification (Commentary)
4	Reaction
2	Reaction Type
16	Reference
5	Specific Activity [micromol/min/mg]
2	Storage Stability
59	Substrates and Products (Substrate)
6	Subunits
13	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
23	Turnover Number [1/s]

Metals Ions

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Metals Ions on EC 2.3.1.18 - galactoside O-acetyltransferase

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

MgSO4

Escherichia coli

-

1 mM, addition to 5,5'-dithiobis(2-nitrobenzoic acid) assay mixture lacking EDTA inhibits, preincubation of transacetylase (2 mg/ml in 0.05 M Tris, pH 7.8) with MgSO4 for 1 h at 30°C and subsequent dilution of the enzyme 1000-fold in 5,5'-dithiobis(2-nitrobenzoic acid) assay mixture lacking both EDTA and the metal ion stimulates

486462

Mn2+

Escherichia coli

-

stimulates when enzyme is initially incubated with the ion in Tris buffer but has no effect if initially incubated in phosphate or if added to the standard assay medium containing 0.05 M phosphate

486460

additional information

Escherichia coli

-

no requirement for divalent cation

486461