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L337F
the point mutation, mutant fab1-1, causes a partially deficient KAS2 activity
E122K
the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides
G200S
the mutant is resistant towards inhibition by acylated sulfonamides
E122K
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the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides
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G200S
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the mutant is resistant towards inhibition by acylated sulfonamides
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C163Q
site-directed mutagenesis, interaction with platensimycin compared to the interaction with the wild-type enzyme
R206G
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R206 impairs the binding of CoA
C164Q
site-directed mutagenesis, a mutant in which the binding site is altered to resemble the substrate-bound state
C164A
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site-directed mutagenesis, inactive mutant
C164A/H337A
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site-directed mutagenesis, inactive mutant
C164A/K332A
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site-directed mutagenesis, inactive mutant
E346A
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site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme
E383A
crystal structure determination and comparison to the wild-type enzyme, the mutation E383A appears to play a key role in disfavouring the less desirable triclinic crystal form and in generating a new surface for a packing interaction that stabilizes the new crystal form
E396A
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site-directed mutagenesis, the mutant shows no condensation activity but retains about 50% of wild-type transacylation activity with acyl-ACP and ACP, and 40% of wild-type decarboxylation activity
H303A
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site-directed mutagenesis, the mutant shows 74% reduced condensation activity, 40% reduced transacylation activity, and 5fold increased decarboxylation activity, compared to the wild-type enzyme
H337A
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site-directed mutagenesis, inactive mutant
K332A
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site-directed mutagenesis, the mutant shows no condensation activity but retains about 30% of wild-type transacylation activity with acyl-ACP and ACP, and 10% of wild-type decarboxylation activity
F107I
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less efficient than wild type protein
F107L
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less efficient than wild type protein
F107S
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suggested that the Ser residue has less significant effects than the Ile and Leu mutations
additional information
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construction of KASII knockout mutants by T-DNA disruption, knockout alleles fab1-1 and fab1-2, strong seed-specific hairpin-RNAi reductions in FAB1 expression resulted in abortion of about 1/4 of the embryos in an apparent phenocopy of fab1-2 homozygosity, in less severe FAB1 hairpin-RNAi individuals, embryos developed normally and exhibited a 1:2:1 segregation ratio for palmitate accumulation, thus, early embryo development appears sensitive to elevated 16:0, whereas at later stages, up to 53% of 16:0, i.e. a 7-fold increase over wild-type levels, is tolerated, Fab1-1 mutant plants show about 60% of wild-type enzyme activity and about 17% palm-like oil accumulation compared to the wild-type plants, phenotypes, overview
additional information
construction of a KAS2 T-DNA insertion mutant, the mutation causes arrest of embryonal development and embryo lethality, phenotype, overview
additional information
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construction of a KAS2 T-DNA insertion mutant, the mutation causes arrest of embryonal development and embryo lethality, phenotype, overview
additional information
transient silencing of the KASII genes in Nicotiana benthamiana for metabolic engineering of wax ester composition, simultaneous inhibition of the two KASII genes Nicotiana benthamiana is possible using three different RNAi constructs
additional information
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expression of Clostridium acetobutylicium FabF1 restores thermal control of fatty acid composition to an Escherichia coli FabF null mutant strain. FabF1 expression leads a modest conversion of cis-3-decenoyl-[acyl-carrier-protein] to trans-2-cis-5-dodecadienoyl-[acyl carrier-protein]. An Escherichia coli fabF- strain in which Clostridium acetobutylicium FabF1 is expressed from the lac promoter of a low copy number vector closely mimicks the changes in fatty acid composition seen in wild type Escherichia coli strains upon changes in growth temperature. Expression of Clostridium acetobutylicium FabF1 restores cis-vaccenate synthesis at all temperatures, but is much more effective at 30°C than at 37°C or 42°C
additional information
enzyme overexpression in a strain deficient in palmitoyl-acyl carrier protein [ACP] thioesterase, EC 3.1.2.14, for improvement of palm oil production in Elaeis guineensis
additional information
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enzyme overexpression in a strain deficient in palmitoyl-acyl carrier protein [ACP] thioesterase, EC 3.1.2.14, for improvement of palm oil production in Elaeis guineensis
additional information
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transient silencing of the KASII genes in Nicotiana benthamiana for metabolic engineering of wax ester composition, simultaneous inhibition of the two KASII genes Nicotiana benthamiana is possible using three different RNAi constructs
additional information
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in vitro complementation of the enzyme-deficient Escherichia coli CY244 strain, shows that Plasmodium falciparum FabB/F functions like Escherichia coli FabF as the growth of the mutant cells can be rescued only in the presence of oleic acid