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A38P
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mutant in 3-O-glucoside-6Â’Â’-malonyltransferase containing regions A and C of anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase, threefold decrease in 3-O-glucoside-6Â’Â’-malonyltransferase activity, anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase activity is 50% of 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase wild-type
A413T
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mutant in 3-O-glucoside-6Â’Â’-malonyltransferase containing regions A and C of anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase, fivefold decrease in 3-O-glucoside-6Â’Â’-malonyltransferase activity, anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase activity is 50% of 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase wild-type
K419N
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mutant in 3-O-glucoside-6Â’Â’-malonyltransferase containing regions A and C of anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase, 50% decrease in 3-O-glucoside-6Â’Â’-malonyltransferase activity, anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase activity is 70% of 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase wild-type
L37V
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mutant in 3-O-glucoside-6Â’Â’-malonyltransferase containing regions A and C of anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase, threefold decrease in 3-O-glucoside-6Â’Â’-malonyltransferase activity, anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase activity similar to 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase wild-type
Q51P
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mutant in 3-O-glucoside-6Â’Â’-malonyltransferase containing regions A and C of anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase, 2.5fold decrease in 3-O-glucoside-6Â’Â’-malonyltransferase activity, anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase activity is 50% of 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase wild-type
T35G
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mutant in 3-O-glucoside-6Â’Â’-malonyltransferase containing regions A and C of anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase, sixfold decrease in 3-O-glucoside-6Â’Â’-malonyltransferase activity, increase in anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase activity
Y411N
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mutant in 3-O-glucoside-6Â’Â’-malonyltransferase containing regions A and C of anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase, ninefold decrease in 3-O-glucoside-6Â’Â’-malonyltransferase activity, no anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase activity
biotechnology
production of anthocyanins to use their anti-oxidative, anti-inflammatory, anti-cancer, anti-obesity, anti-diabetic, and cardioprotective properties
medicine
production of anthocyanins to use their anti-oxidative, anti-inflammatory, anti-cancer, anti-obesity, anti-diabetic, and cardioprotective properties
synthesis
production of anthocyanins to use their anti-oxidative, anti-inflammatory, anti-cancer, anti-obesity, anti-diabetic, and cardioprotective properties
additional information
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swapping of regions A, B and C of anthocyanidin 3-O-glucoside-6Â’Â’-malonyltransferase and anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase and identification of amino acid residues that are important for anthocyanidin 3-O-glucoside-3Â’Â’,6Â’Â’-dimalonyltransferase activity in regions A and C