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2.3.1.17: aspartate N-acetyltransferase

This is an abbreviated version!
For detailed information about aspartate N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.17

Reaction

acetyl-CoA
+
L-aspartate
=
CoA
 +
N-acetyl-L-aspartate

Synonyms

acetyltransferase, aspartate, ANAT, aspartate acetyltransferase, aspartate N-acetyltransferase, aspartic acetylase, L-aspartate N-acetyltransferase, NAT, NAT8L

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.17 aspartate N-acetyltransferase

Cloned

Cloned on EC 2.3.1.17 - aspartate N-acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression HEK-293 cell
expression in HEK-293T cell
gene nat8l, sequence comparisons, subcloning of the genes for thioredoxin (TRX), glutathione S-transferase (GST) or maltose binding protein (MBP), followed by a linker region (21-68-amino acids) containing various cleavage site sequences, and then connected to the N-terminal of the nat8l gene, without the membrane anchor, recombinant functional and soluble expression of the dual affinity tagged enzyme as MBP-fusion protein in Escherichia coli strain BL21(DE3), method evaluation
overexpressed both as native and as fusion protein with a His6 tag at the C- or the N-terminus in human embryonic kidney-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T cells)
overexpressed both as native and as fusion proteins with a His6 tag at the C- or the N-terminus in human embryonic kidney-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T cells); furthermore expressed as fusion protein in rat embryonal cortical neurons and in Chinese-hamster ovary cells
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