2.3.1.169: CO-methylating acetyl-CoA synthase
This is an abbreviated version!
For detailed information about CO-methylating acetyl-CoA synthase, go to the full flat file.
Word Map on EC 2.3.1.169
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2.3.1.169
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methanosarcina
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thermophila
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acetylthiocholine
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methyl-coenzyme
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methanogenesis
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butyrylthiocholine
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busch
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base-off
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propionylthiocholine
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nickel-containing
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moorella
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azinphos-methyl
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acetivorans
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2-naphthyl
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thermoacetica
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bioorganometallic
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five-subunit
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methylcobalamin
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acetate-grown
- 2.3.1.169
- methanosarcina
- thermophila
- acetylthiocholine
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methyl-coenzyme
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methanogenesis
- butyrylthiocholine
-
busch
-
base-off
- propionylthiocholine
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nickel-containing
-
moorella
- azinphos-methyl
- acetivorans
-
2-naphthyl
- thermoacetica
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bioorganometallic
-
five-subunit
- methylcobalamin
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acetate-grown
Reaction
Synonyms
ACDS multienzyme complex, acetyl-CoA decarbonylase/synthase multienzyme complex, acetyl-CoA decarboxylase/synthase, Acetyl-CoA synthase, Acetyl-coenzyme A synthase, acetyl-coenzyme A synthase/carbon monoxide dehydrogenase, ACS/CODH, ACSCh, Carbon monoxide dehydrogenase, carbon monoxide dehydrogenase-corrinoid enzyme complex, carbon monoxide dehydrogenase/acetyl coenzyme A synthase, carbon monoxide dehydrogenase/acetyl-CoA synthase, carbon monoxide dehydrogenase/acetyl-coenzyme A synthase, CDH1, Cdh2, CO dehydrogenase, CO dehydrogenase enzyme complex, CO dehydrogenase/acetyl CoA synthase, CO dehydrogenase/acetyl coenzyme A synthase, CO dehydrogenase/acetyl-CoA synthase, CODH, CODH/ACS, CODH/ASC, KSU1_D0226, KSU1_D0227, monoxide dehydrogenase/acetyl-CoA synthase, multienzyme carbon monoxide dehydrogenase complex, multienzyme CO dehydrogenase/acetyl-CoA synthase complex
ECTree
2.3.1.169 CO-methylating acetyl-CoA synthaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.3.1.169 - CO-methylating acetyl-CoA synthase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
CH3-CO-S-CoA + corrinoid/iron-sulfur protein
CH3-CO-S-CoA + tetrahydrosarcinapterin + H2O
CH3-tetrahydrosarcinapterin + CO2 + H+ + electron
CH3-tetrahydrosarcinapterin + CO + HS-CoA
CH3-CO-S-CoA + tetrahydrosarcinapterin
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-
-
?
acetyl-CoA + corrinoid protein
CoA + CO + methylcorrinoid protein
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-
-
-
r
acetyl-CoA + corrinoid protein
CoA + CO + methylcorrinoid protein
-
-
-
-
r
acetyl-CoA + corrinoid protein
CoA + CO + methylcorrinoid protein
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-
-
-
r
acetyl-CoA + corrinoid protein
CoA + CO + methylcorrinoid protein
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-
-
-
r
acetyl-CoA + corrinoid protein
CoA + CO + methylcorrinoid protein
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-
-
-
?
acetyl-CoA + corrinoid protein
CoA + CO + methylcorrinoid protein
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Fd-II can act as a redox mediator by accepting electrons from the acetyl-ACS intermediate and by serving as the initial reducing agent linked to formation of the Ni1+-CO catalytic intermediate
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-
?
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
CH3-CO-S-CoA + corrinoid/iron-sulfur protein
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-
-
-
?
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
CH3-CO-S-CoA + corrinoid/iron-sulfur protein
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-
-
-
?
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
CH3-CO-S-CoA + corrinoid/iron-sulfur protein
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-
-
?
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
CH3-CO-S-CoA + corrinoid/iron-sulfur protein
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under anaerobic conditions
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?
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
CH3-CO-S-CoA + corrinoid/iron-sulfur protein
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under anaerobic conditions
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?
CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA
CH3-CO-S-CoA + corrinoid/iron-sulfur protein
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under anaerobic conditions
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-
?
CH3-CO-S-CoA + H+ + tetrahydromethanopterin
CH3-tetrahydromethanopterin + CO + HS-CoA
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-
?
CH3-CO-S-CoA + H+ + tetrahydromethanopterin
CH3-tetrahydromethanopterin + CO + HS-CoA
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-
?
CH3-CO-S-CoA + tetrahydrosarcinapterin + H2O
CH3-tetrahydrosarcinapterin + CO2 + H+ + electron
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the carbon monoxide dehydrogenase-corrinoid enzyme complex catalyzes the cleavage of acetyl-CoA, tetrahydrosarcinapterin functions as the methyl group acceptor, the major products of reaction are methyltetrahydrosarcinapterin and CO2, free CoA is identified as an additional product
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?
CH3-CO-S-CoA + tetrahydrosarcinapterin + H2O
CH3-tetrahydrosarcinapterin + CO2 + H+ + electron
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the carbon monoxide dehydrogenase-corrinoid enzyme complex catalyzes the cleavage of acetyl-CoA, tetrahydrosarcinapterin functions as the methyl group acceptor, the major products of reaction are methyltetrahydrosarcinapterin and CO2, free CoA is identified as an additional product
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?
CH3-tetrahydrofolate + CO + HS-CoA
CH3-CO-S-CoA + tetrahydrofolate
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-
-
?
CH3-tetrahydrofolate + CO + HS-CoA
CH3-CO-S-CoA + tetrahydrofolate
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-
-
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?
CH3-tetrahydrofolate + CO + HS-CoA
CH3-CO-S-CoA + tetrahydrofolate
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-
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?
CH3-tetrahydrofolate + CO + HS-CoA
CH3-CO-S-CoA + tetrahydrofolate
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this multistep reaction involves four proteins: CO dehydrogenase, methyltransferase, the corrinoid/iron-sulfur protein and ferredoxin
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?
CH3-tetrahydrofolate + CO + HS-CoA
CH3-CO-S-CoA + tetrahydrofolate
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The methyltransferase catalyses the reaction of CH3-H4folate with the corrinoid/iron-sulfur protein to form a methylcobalt species. The Ni/Fe-S enzyme CO dehydrogease then catalyses the final steps in the formation of acetyl-CoA.
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?
CH3I + CO + HS-CoA
CH3-CO-S-CoA + HI
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the multienzyme complex catalyses the acetyl-CoA synthesis from CH3I, CO and CoA as well as to cleave acetyl-CoA into its methyl, carbonyl, and CoA components as the first step in the catabolism of acetyl-CoA to methane and CO2
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?
CO + H2O
CO2 + H+ + electron
the multienzyme complex catalyses the reversible oxidation of CO to CO2
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r
CO + H2O
CO2 + H+ + electron
the multienzyme complex catalyses the reversible oxidation of CO to CO2
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r
CO + H2O
CO2 + H+ + electron
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the multienzyme complex catalyses the reversible oxidation of CO to CO2
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r
CO + H2O
CO2 + H+ + electron
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the NiFe4S4-5C cluster catalyses the reversible oxidation of CO to CO2
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r
CO + methyl-X + HS-CoA
CH3-CO-S-CoA + HX
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acetyl-CoA synthase catalyses acetyl-CoA synthesis, an intermediate step is the transfer of the cobalt-bound methyl group from methylated corrinoid/iron-sulfur protein to the acetyl-CoA synthase
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?
CO + methyl-X + HS-CoA
CH3-CO-S-CoA + HX
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acetyl-CoA synthase catalyses acetyl-CoA synthesis, an intermediate step is the transfer of the cobalt-bound methyl group from methylated corrinoid/iron-sulfur protein to the acetyl-CoA synthase
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?
CO2 + H+ + electron
CO + H2O
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CO dehydrogenase catalyses the two-electron reduction of CO2 to CO
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?
CO2 + H+ + electron
CO + H2O
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CO dehydrogenase catalyses the two-electron reduction of CO2 to CO
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?
additional information
?
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the enzyme catalyzes the exchange of 14C from the carboxyl group of acetyl-CoA with 12C from CO
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?
additional information
?
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an Fe/S-containing active site metal center, the A cluster, catalyzes acetyl CC bond formation/breakdown. Carbonyl group exchange of acetyl-CoA with CO is a hallmark of CODH/ACS, coupling analysis of the recombinant A cluster protein of acetyl-CoA synthase of Carboxydothermus hydrogenoformans, ACSCh, and truncated ACSCh lacking its 317-amino acid N-terminal domain, overview
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?
additional information
?
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in assays of bacterial ACS, methylated corrinoid iron-sulfur protein is generally used as the source of methyl groups for acetyl-CoA synthesis
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?
additional information
?
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an Fe/S-containing active site metal center, the A cluster, catalyzes acetyl CC bond formation/breakdown. Carbonyl group exchange of acetyl-CoA with CO is a hallmark of CODH/ACS, coupling analysis of the recombinant A cluster protein of acetyl-CoA synthase of Carboxydothermus hydrogenoformans, ACSCh, and truncated ACSCh lacking its 317-amino acid N-terminal domain, overview
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?
additional information
?
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methylcobinamide, methylcobalamin, and CH3-(Me3-benzimidazolyl)cobamide are substrates of the acetyl-CoA synthase, methylcobalamin is 2000fold less reactive than methylcobinamide, CO dehydrogenase catalyses the CO-dependent reduction of methylcobinamide 10000fold faster than that of methylcobalamin
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?
additional information
?
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the bifuctional enzyme CO dehydrogenase/acetyl-CoA synthase is central to the Wood-Ljungdahl pathway of autotrophic CO2 fixation
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?
additional information
?
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the multienzyme complex catalyses the exchange between free CO and carbonyl group of acetyl-CoA, and the exchange between CoA and the CoA moiety of acetyl-CoA
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-
?
additional information
?
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the multienzyme complex catalyses the exchange between free CO and carbonyl group of acetyl-CoA, and the exchange between CoA and the CoA moiety of acetyl-CoA
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-
?
additional information
?
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an nickel-containing active site metal center, the A cluster, catalyzes acetyl C-C bond formation/breakdown. Carbonyl group exchange of acetyl-CoA with CO is weakly active in ACDS, and exchange with CO2 is up to 350 times faster, indicating tight coupling of CO release at the A cluster to CO oxidation to CO2 at the C cluster in CO dehydrogenase, coupling analysis of the recombinant A cluster protein of ACDS. Direct role of the ACS N-terminal domain in promoting acetyl C-C bond fragmentation. Protein conformational changes, related to open/closed states have direct effects on the coordination geometry and stability of the A cluster Ni2+-acetyl intermediate, controlling Ni2-acetyl fragmentation and Ni2(CO)(CH3) condensation. Involvement of subunit-subunit interactions in ACDS, versus interdomain contacts in ACS, ensures that CO is not released from the ACDS beta-subunit in the absence of appropriate interactions with the alpha2epsilon2 CO dehydrogenase component, ACDS complex partial reactions in the overall synthesis and cleavage of acetyl-CoA, overview
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-
?
additional information
?
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an nickel-containing active site metal center, the A cluster, catalyzes acetyl C-C bond formation/breakdown. Carbonyl group exchange of acetyl-CoA with CO is weakly active in ACDS, and exchange with CO2 is up to 350 times faster, indicating tight coupling of CO release at the A cluster to CO oxidation to CO2 at the C cluster in CO dehydrogenase, coupling analysis of the recombinant A cluster protein of ACDS. Direct role of the ACS N-terminal domain in promoting acetyl C-C bond fragmentation. Protein conformational changes, related to open/closed states have direct effects on the coordination geometry and stability of the A cluster Ni2+-acetyl intermediate, controlling Ni2-acetyl fragmentation and Ni2(CO)(CH3) condensation. Involvement of subunit-subunit interactions in ACDS, versus interdomain contacts in ACS, ensures that CO is not released from the ACDS beta-subunit in the absence of appropriate interactions with the alpha2epsilon2 CO dehydrogenase component, ACDS complex partial reactions in the overall synthesis and cleavage of acetyl-CoA, overview
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-
?
additional information
?
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the multienzyme complex catalyses the exchange between free CO and carbonyl group of acetyl-CoA, and the exchange between CoA and the CoA moiety of acetyl-CoA
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-
?
additional information
?
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enzyme catalyses the CoA/acetyl-CoA exchange
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?
additional information
?
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methylcobinamide, methylcobalamin, and CH3-(Me3-benzimidazolyl)cobamide are substrates of the acetyl-CoA synthase, methylcobalamin is 2000fold less reactive than methylcobinamide, CO dehydrogenase catalyses the CO-dependent reduction of methylcobinamide 10000fold faster than that of methylcobalamin
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-
?
additional information
?
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key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway
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?
additional information
?
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key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway
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?
additional information
?
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key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway
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?
additional information
?
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key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway
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?
additional information
?
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key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway
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-
?
additional information
?
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key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway
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-
?
additional information
?
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key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway
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-
?
additional information
?
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enzyme and a corrinoid/iron-sulfur protein, methyltransferase and an electron transfer protein such as ferredoxin II play a pivotal role in the conversion of methylhydrofolate, CO, and CoA to acetyl-CoA
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?
additional information
?
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the bifuctional enzyme CO dehydrogenase/acetyl-CoA synthase is central to the Wood-Ljungdahl pathway of autotrophic CO2 fixation
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-
?
additional information
?
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CoA is the last substrate to bind and CO and the methyl group bind randomly as the first substrate in acetyl-CoA synthesis. In pulse-chase experiments, up to 100% of the methyl groups and CoA and up to 60-70% of the CO employed in the pulse phase can be trapped in the product acetyl-CoA
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?
additional information
?
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the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO
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?
additional information
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mechanism by which acetyl-CoA is assembled at the A-cluster and mechanism of CO2 reduction at the C-cluster, overview
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?
