2.3.1.169: CO-methylating acetyl-CoA synthase

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For detailed information about CO-methylating acetyl-CoA synthase, go to the full flat file.

Reaction

Synonyms

ACDS multienzyme complex, acetyl-CoA decarbonylase/synthase multienzyme complex, acetyl-CoA decarboxylase/synthase, Acetyl-CoA synthase, Acetyl-coenzyme A synthase, acetyl-coenzyme A synthase/carbon monoxide dehydrogenase, ACS/CODH, ACSCh, Carbon monoxide dehydrogenase, carbon monoxide dehydrogenase-corrinoid enzyme complex, carbon monoxide dehydrogenase/acetyl coenzyme A synthase, carbon monoxide dehydrogenase/acetyl-CoA synthase, carbon monoxide dehydrogenase/acetyl-coenzyme A synthase, CDH1, Cdh2, CO dehydrogenase, CO dehydrogenase enzyme complex, CO dehydrogenase/acetyl CoA synthase, CO dehydrogenase/acetyl coenzyme A synthase, CO dehydrogenase/acetyl-CoA synthase, CODH, CODH/ACS, CODH/ASC, KSU1_D0226, KSU1_D0227, monoxide dehydrogenase/acetyl-CoA synthase, multienzyme carbon monoxide dehydrogenase complex, multienzyme CO dehydrogenase/acetyl-CoA synthase complex

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.169 CO-methylating acetyl-CoA synthase

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Results	in table
5	Activating Compound
2659	AA Sequence
2	CAS Registry Number
8	Cloned(Commentary)
3	Cofactor
6	Crystallization (Commentary)
1	Expression
23	General Information
2	General Stability
21	Inhibitors
1	kcat/KM [mM/s]
2	Ki Value [mM]
11	KM Value [mM]
1	Localization
62	Metals/Ions
11	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
60	Organism
7	Pathway
48	PDB ID
13	pH Optimum
11	Protein Variants
7	Purification (Commentary)
32	Reaction
1	Reaction Type
47	Reference
1	Renatured (Commentary)
1	Source Tissue
7	Specific Activity [micromol/min/mg]
66	Substrates and Products (Substrate)
13	Subunits
42	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 2.3.1.169 - CO-methylating acetyl-CoA synthase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

native enzyme by anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography, gel filtration, and CoA affinity chromatography

Moorella thermoacetica

-

390459

recombinant ACSCh732 and ACSChDELTAN from Escherichia coli strain NM522 by anion exchange and hydroxyapatite chromatography, followed by another and different step of anion exchange chromatography and by hydrophobic interaction chromatography to 94-95% purity forACSCh and around 98% purity for ACSChDELTAN

Carboxydothermus hydrogenoformans

-

719862

recombinant ACSD beta from Escherichia coli strain NM522 by anion exchange chromatography, followed by hydrophobic interaction chromatography and another and different step of ion exchange of anion exchange chromatography to around 98% purity for ACDS beta

Methanosarcina thermophila

-

719862

recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography under a N2 atmosphere

Moorella thermoacetica

-

714240

-

Methanosarcina barkeri

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390474

-

Moorella thermoacetica

-

644674, 644850