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2.3.1.169: CO-methylating acetyl-CoA synthase

This is an abbreviated version!
For detailed information about CO-methylating acetyl-CoA synthase, go to the full flat file.

Word Map on EC 2.3.1.169

Reaction

acetyl-CoA
+
a [Co(I) corrinoid Fe-S protein]
=
CO
+
CoA
+
a [methyl-Co(III) corrinoid Fe-S protein]

Synonyms

ACDS multienzyme complex, acetyl-CoA decarbonylase/synthase multienzyme complex, acetyl-CoA decarboxylase/synthase, Acetyl-CoA synthase, Acetyl-coenzyme A synthase, acetyl-coenzyme A synthase/carbon monoxide dehydrogenase, ACS/CODH, ACSCh, Carbon monoxide dehydrogenase, carbon monoxide dehydrogenase-corrinoid enzyme complex, carbon monoxide dehydrogenase/acetyl coenzyme A synthase, carbon monoxide dehydrogenase/acetyl-CoA synthase, carbon monoxide dehydrogenase/acetyl-coenzyme A synthase, CDH1, Cdh2, CO dehydrogenase, CO dehydrogenase enzyme complex, CO dehydrogenase/acetyl CoA synthase, CO dehydrogenase/acetyl coenzyme A synthase, CO dehydrogenase/acetyl-CoA synthase, CODH, CODH/ACS, CODH/ASC, KSU1_D0226, KSU1_D0227, monoxide dehydrogenase/acetyl-CoA synthase, multienzyme carbon monoxide dehydrogenase complex, multienzyme CO dehydrogenase/acetyl-CoA synthase complex

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.169 CO-methylating acetyl-CoA synthase

Engineering

Engineering on EC 2.3.1.169 - CO-methylating acetyl-CoA synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A110C
A219F
-
mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed
A222L
A265M
-
absence of strong cooperative inhibition of CO which characterizes wild-type enzyme
A578C
-
mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed
F70W
-
mutant designed to block the region that connects the tunnel at the betabeta interface with a water channel also located at the interface. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed
L215F
-
mutant designed to block the tunnel through which CO and CO2 migrate. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed
N101Q
-
mutant designed to block the region that connects the tunnel at the betabeta interface with a water channel also located at the interface. Metal clusters are properly assembled but only slowly reducible by CO. Mutant shows impaired ability of CO to migrate through the tunnel to the C-cluster and reduced catalytic activity, no cooperative CO inhibition is observed
additional information
-
construction of several cdh gene knockouts, genotypic and phenotypic analysis of cdh mutants. CODH activity during aceticlastic and carboxidotrophic growth is reduced in the cdh1 mutant (strain MCD1) compared to the wild-type