2.3.1.164: isopenicillin-N N-acyltransferase

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For detailed information about isopenicillin-N N-acyltransferase, go to the full flat file.

Reaction

Synonyms

6-aminopenicillanate acyltransferase, 6-aminopenicillanic acid acyltransferase, 6-aminopenicillinanic acid phenylacetyltransferase, 6-aminopenicillinanic acylase, aatA, aatB, acyl coA:isopenicillin N-acyltransferase, acyl coenzyme A: isopenicillin N acyltransferase, acyl coenzyme A:6-aminopenicillanic acid acyltransferase, acyl-CoA:6-aminopenicillanate acyltransferase, acyl-CoA:6-APA acyltransferase, acyl-CoA:isopenicillin N acyltransferase, acyl-coenzyme A: IPN acyltransferase, acyl-coenzyme A:isopenicillin N acyltransferase, acyl-coenzyme A:isopenicillin N-acyltransferase, acyltransferase, 6-aminopenicillanate, IAT, IPN acyltransferase, isopenicillin N acyltransferase, isopenicillin N:acyl CoA acyltransferase, isopenicillin N:acyl-CoA: acyltransferase, isopenicillin-N N-acyltransferase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.164 isopenicillin-N N-acyltransferase

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Results	in table
39	AA Sequence
3	Application
1	CAS Registry Number
10	Cloned(Commentary)
2	Crystallization (Commentary)
7	Expression
7	General Information
7	Localization
19	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
29	Organism
4	Pathway
12	PDB ID
1	pH Optimum
4	Posttranslational Modification
9	Protein Variants
3	Purification (Commentary)
1	Reaction
6	Reaction Type
25	Reference
3	Source Tissue
1	Specific Activity [micromol/min/mg]
13	Substrates and Products (Substrate)
11	Subunits
37	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Cloned

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Cloned on EC 2.3.1.164 - isopenicillin-N N-acyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli JM109

Penicillium chrysogenum

-

441302, 441304, 441306, 441307

expression in Escherichia coli

Penicillium chrysogenum

-

660448

gene penDE

Penicillium chrysogenum

P15802

720282

gene penDE, lack of correlation between the protein and transcript levels of the IAT enzyme, quantitative real-time PCR expression analysis and gene copy number determination, overexpression of penDE in Penicillium chrysogenum strains DS47274, DS47273, and DS17690, introduction of penDE gene without introns into these strains in a random manner, via the nonhomologous end-joining pathway. Cooverexpression of penDE with gene phl, encoding the phenylacetic acid CoA ligase leading to production of phenoxyacetyl-CoA and penicillin V

Penicillium chrysogenum

-

718653

overexpression in Escherichia coli, not self-processed even when treated with Aspergillus nidulans crude extract

Aspergillus nidulans

-

658618

pPenIAT and pPenIATC103S are used to express the penDE gene in Penicillium chrysogenum, pPBCalphabeta, pDEC103S and palphabeta-DEC103S are used for expression in Escherichia coli

Penicillium chrysogenum

-

686918

the intronless gene is expressed in Escherichia coli and self-processed efficiently into its alpha and beta subunits

Penicillium chrysogenum

-

658618