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2.3.1.151: 2,3',4,6-tetrahydroxybenzophenone synthase

This is an abbreviated version!
For detailed information about 2,3',4,6-tetrahydroxybenzophenone synthase, go to the full flat file.

Word Map on EC 2.3.1.151

Reaction

3 Malonyl-CoA +

3-hydroxybenzoyl-CoA
= 4 CoA +
2,3',4,6-tetrahydroxybenzophenone
 + 3 CO2

Synonyms

2,3',4,6-Tetrahydroxybenzophenone synthase, benzophenone synthase, BPS, More, Synthase, benzophenone

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.151 2,3',4,6-tetrahydroxybenzophenone synthase

Engineering

Engineering on EC 2.3.1.151 - 2,3',4,6-tetrahydroxybenzophenone synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A257G
site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme
G339S
site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme
G339V
site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme
T133L
site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme
T135A
-
inactive enzyme
T135F
-
mutant functionally resemble the wild-type enzyme, albeit with reduced catalytic activities
T135G
-
inactive enzyme
T135I
-
inactive enzyme
T135L
-
a point mutation in the active site cavity transforms benzophenone synthase into a functional phenylpyrone synthase (PPS). The dramatic change in both substrate and product specificities of benzophenone synthase is rationalized by homology modeling. The mutation may open a new pocket that accommodates the phenyl moiety of the triketide intermediate but limits polyketide elongation to two reactions, resulting in phenylpyrone formation. kcat (substrate: benzoyl-CoA): 0.0086/sec, Km (benzoyl-CoA): 0.001 mM, Km (malonyl-CoA): 8.7 mM. In contrast to the wild-type enzyme, the T135L mutant forms phenylpyrone as a major product and only traces of 2,4,6-trihydroxybenzophenone when incubated with benzoyl-CoA and malonyl-CoA. T135L mutant is almost inactive with 3-hydroxybenzoyl-CoA
T135N
-
inactive enzyme
T135S
-
mutant functionally resemble the wild-type enzyme, albeit with reduced catalytic activities
T135V
-
inactive enzyme
T135Y
-
inactive enzyme