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2.3.1.15: glycerol-3-phosphate 1-O-acyltransferase

This is an abbreviated version!
For detailed information about glycerol-3-phosphate 1-O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.15

Reaction

an acyl-CoA
+
sn-glycerol 3-phosphate
=
CoA
 +
a 1-acyl-sn-glycerol 3-phosphate

Synonyms

1-acyl-sn-glycerol-3-phosphate acyltransferase, 3-glycerophosphate acyltransferase, ACP:sn-glycerol-3-phosphate acyltransferase, acyl-CoA:glycerol-3-phosphate acyltransferase 1, acyl-CoA:sn-glycerol-3-phosphate O-acyltransferase, acyl-[acyl carrier protein]:sn-1-glycerol-3-phosphate acyltransferase, acyltransferase, glycerol phosphate, Ad-GPAT1, AGPAT, AGPAT10, AGPAT6, AGPAT8, AGPAT9, alpha-glycerophosphate acyltransferase, AT1G02390, AT1G06520, AT3G11430, AT4G00400, AT4G01950, AT5G06090, AT5G60620, AtGPAT1, AtGPAT2, AtGPAT3, AtGPAT5, AtGPAT7, AtGPAT8, AtGPAT9, cancer-testicular antigen, chloroplast glycerol-3-phosphate acyltransferase, CrGAPTcl, ER-associated GPAT, G3P acyltransferase, GAT, Gat1, GAT1p, GAT2p, glycerol 3-phosphate acyltransferase, glycerol phosphate acyltransferase, glycerol phosphate transacylase, glycerol-3-P acyltransferase 1, glycerol-3-phosphate acyltransferase, glycerol-3-phosphate acyltransferase 1, glycerol-3-phosphate acyltransferase 2, glycerol-3-phosphate acyltransferase 3, glycerol-3-phosphate acyltransferase 4, glycerol-3-phosphate acyltransferase 9, glycerol-3-phosphate acyltransferase-1, glycerol-3-phosphate acyltransferase-like protein 1, glycerol-3-phosphate O-acyltransferase, glycerol-3-phosphate-1 acyltransferase, glycerol-sn-3-phosphate acyltransferase 1, glycerophosphate acyltransferase, glycerophosphate transacylase, GPAM, GPAT, GPAT-1, GPAT-4, GPAT-9, GPAT1, GPAT2, GPAT3, GPAT3/AGPAT10, GPAT4, GPAT5, GPAT6, GPAT7, GPAT8, GPAT9, gpt1, Gpt2, LeGPAT, LiGPAT, LPAAT-theta, LPAAT-zeta, mGPAT, microsomal GPAT3, microsomal GPAT3/AGPAT10, microsomal GPAT4, mitochondrial glycerol-3-phosphate acyltransferase, mitochondrial GPAT1, mitochondrial GPAT2, More, MS33, mtGPAT, mtGPAT1, mtGPAT2, OsGPAT3, PHATRDRAFT_42446, PHYPADRAFT_137573, PLAT2, PlsB, PlsB1, Ppa GPAT9, PpGPAT9, PtGPAT, Rv1551, Rv1551 acyltransferase, SA1, Sn-glycerol 3-phosphate acyltransferase, sn-glycerol-3-phosphate acyltransferase, SsGPAT, TbGAT, water-soluble plastidial glycerol-3-phosphate acyltransferase, xGPAT1, xGPAT1-v1, xGPAT1-v2

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.15 glycerol-3-phosphate 1-O-acyltransferase

Purification

Purification on EC 2.3.1.15 - glycerol-3-phosphate 1-O-acyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoforms of different pI-values
-
affinity chromatography on 6-phosphogluconic acid-Sepharose 4B
-
ammonium sulfate, DEAE-cellulose, Sephacryl S-300
-
ammonium sulfate, Sephadex G-100, DEAE-Trisacryl, Mono Q
-
by anti-FLAG M2 affinity chromatography
-
enzyme is isolated using subcellular fractions
-
GPAT1 from rat is purified to homogeneity
heparin-Sepharose, Yellow 86-Sepharose, Superdex 200, hydroxylapatite
-
high solubilisation is achieved using 0.75% Tween80 and the solubilised GPAT is partially purified by ion-exchange chromatography using a Hi-Trap DEAE FF column, followed by gel filtration chromatography using a Superose 12 HR column
-
His SpinTrap column chromatography
isoenzyme AT1, DEAE-Toyopearl, [acyl-carrier protein]-affinity chromatography, MonoP
Q9FEP9, Q9FEQ0
isoenzyme AT1, [acyl-carrier protein]-affinity chromatography, anion-exchange column, isoenzymes AT2 and AT3, [acyl-carrier protein]-affinity chromatography, hydroxylapatite
-
isozymes partially by preparation of subcellular fractions
-
liver mitochondria are prepared
-
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite
-
microsomal enzyme, Sepharose 2B, sucrose gradient, partial purification
-
mitochondria and submitochondrial fractions are purified by gradient centrifugation, radiolabeled GPAT1 is synthesized by an in vitro transcription-translation system
-
mitochondria are isolated from rat liver by differential centrifugation
mitochondrial acyltransferase, Bio-Gel HAT, Rotofor, Sephacryl S-300
-
mitochondrial enzyme, cholate, ammonium sulfate, partial purification
-
mitochondrial enzyme, Sepharose Q, BioGel HAT, octyl-Sepharose CL-4B, partial purification
-
partial purification
-
partial purification of microsomal acyl transferase on glycerophosphorylethanolamine affinity column
-
partial, Tween-80/NaCl-solubilized
recombinant acyltransferase, Mono Q
recombinant chimeric mature enzyme
-
recombinant His-tagged acyl transferase, Ni2+-affinity column
-
recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography and ultrafiltration
recombinant native and selenomethionine-labeled wild-type and chimeric mature enzymes by anio exchange chromatography
-
solubilized, by non-ionic detergents
-
solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles
-
to determine the translated size of GPAT2, in vitro transcription and translation is performed
-
Triton X-100-solubilized
using a nickel gelose gel FF column