2.3.1.137: carnitine O-octanoyltransferase

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For detailed information about carnitine O-octanoyltransferase, go to the full flat file.

Reaction

Synonyms

carnitine acyltransferase, Carnitine medium-chain acyltransferase, carnitine octanoyltransferase, COT, CRAT, CrOT, easily solubilized mitochondrial carnitine palmitoyltransferase, medium-chain carnitine acyltransferase, medium-chain/long-chain carnitine acyltransferase, More, overt mitochondrial carnitine palmitoyltransferase, short-chain carnitine acyltransferase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.137 carnitine O-octanoyltransferase

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Results	in table
1	Activating Compound
21	AA Sequence
1	CAS Registry Number
8	Cloned(Commentary)
1	Crystallization (Commentary)
42	Disease/ Diagnostics
1	General Information
2	General Stability
35	Inhibitors
5	Ki Value [mM]
63	KM Value [mM]
84	Localization
13	Molecular Weight [Da]
131	Natural Substrates/ Products (Substrates)
563	Organism
8	PDB ID
3	pH Optimum
2	pH Range
9	Protein Variants
14	Purification (Commentary)
4	Reaction
3	Reaction Type
64	Reference
75	Source Tissue
3	Specific Activity [micromol/min/mg]
6	Storage Stability
304	Substrates and Products (Substrate)
9	Subunits
46	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Engineering

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Engineering on EC 2.3.1.137 - carnitine O-octanoyltransferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

R518N

Bos taurus

-

site-directed mutagenesis, mutant shows 1650fold increased Km for L-carnitine, only slightly affected Km for acyl-CoA and kcat compared to the wild-type enzyme

657546

S552A

Bos taurus

-

site-directed mutagenesis, mutant shows 17fold increased Km for L-carnitine compared to the wild-type enzyme

657546

S554X

Bos taurus

-

site-directed mutagenesis, mutant shows 10fold decreased kcat compared to the wild-type enzyme

657546

C323M

Mus musculus

Q9DC50

site-directed mutagenesis, crystal structure comparison with the wild-type enzyme, altered acyl-CoA substrate specificity compared to the wild-type enzyme, highly increased specificity for octanoyl-CoA

659511

G553M

Mus musculus

Q9DC50

site-directed mutagenesis, highly reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme

659511

M335A

Mus musculus

Q9DC50

site-directed mutagenesis, reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme

659511

M335V

Mus musculus

Q9DC50

site-directed mutagenesis, crystal structure comparison with the wild-type enzyme, highly reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme

659511

G553M

Rattus norvegicus

-

site-directed mutagenesis, the mutant enzyme shows altered substrate specificity: highly reduced activity with medium- and long-chain acyl-CoAs and increased activity with acetyl-CoA and butanoyl-CoA compared to the wild-type enzyme

659372

additional information

Rattus norvegicus

-

the carnitine acetyl-transferase, specific for acetyl-CoA/short-chain acyl-CoAs, can be modified to an enzyme with elevated carnitine octanoyltransferase activity by mutation of Met564 to Gly, overview

659372