2.3.1.129: acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase
This is an abbreviated version!
For detailed information about acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase, go to the full flat file.
Word Map on EC 2.3.1.129
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2.3.1.129
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udp-glcnac
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lipopolysaccharide
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left-handed
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acyl-acp
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endotoxin
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drug development
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beta-helix
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beta-helical
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homotrimer
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udp-3-o-r-3-hydroxymyristoyl-glcnac
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raetz
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medicine
- 2.3.1.129
- udp-glcnac
- lipopolysaccharide
-
left-handed
- acyl-acp
- endotoxin
- drug development
-
beta-helix
-
beta-helical
-
homotrimer
-
udp-3-o-r-3-hydroxymyristoyl-glcnac
-
raetz
- medicine
Reaction
Synonyms
acyltransferase, uridine diphosphoacetylglucosamine, LiLpxA, LpxA, type II ACP-dependent UDP-N-acetylglucosamine acyltransferase, type II acyl carrier protein-dependent UDP-N-acetylglucosamine acyltransferase, UDP-N-acetylglucosamine 3-O-acyltransferase, UDP-N-acetylglucosamine acyltransferase, uridine diphosphoacetylglucosamine acyltransferase
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Purification
Purification on EC 2.3.1.129 - acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase
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recombinant LpxA from Echerichia coli strain BL21-AI/pET24a by Reactive Green 19 affinity chromatography, dialysis, and ion exchange chromatography
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recombinant LpxA, excluding the region encoding the predicted signal peptide (residues 2-32), from Escherichia coli strain C41(DE3) by Reactive Green 19 affinity chromatography and gel filtration to homogeneity
using Ni-NTA chromatography and gel filtration. Around 15 mg of purified protein is obtained from 1 l of bacterial culture