2.3.1.122: trehalose O-mycolyltransferase
This is an abbreviated version!
For detailed information about trehalose O-mycolyltransferase, go to the full flat file.
Word Map on EC 2.3.1.122
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2.3.1.122
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tuberculosis
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mycobacterium
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bovis
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esat-6
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antigen-specific
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ifn-gamma
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bacille
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ankara
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prime-boost
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mva85a
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immunodominant
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mycolylation
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bcg-vaccinated
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mycolic
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tuberculin
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booster
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polyfunctional
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aerosol
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bcg-primed
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calmette-guerin
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elispot
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drug development
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th1-type
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fibronectin-binding
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bcg-induced
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medicine
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tuberculosis-infected
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biotechnology
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dimycolate
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post-vaccination
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ifn-gamma-producing
- 2.3.1.122
- tuberculosis
- mycobacterium
- bovis
-
esat-6
-
antigen-specific
- ifn-gamma
-
bacille
- ankara
-
prime-boost
-
mva85a
-
immunodominant
-
mycolylation
-
bcg-vaccinated
-
mycolic
-
tuberculin
-
booster
-
polyfunctional
-
aerosol
-
bcg-primed
- calmette-guerin
-
elispot
- drug development
-
th1-type
-
fibronectin-binding
-
bcg-induced
- medicine
-
tuberculosis-infected
- biotechnology
-
dimycolate
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post-vaccination
-
ifn-gamma-producing
Reaction
2 alpha,alpha-trehalose 6-mycolate
=
Synonyms
Ag85A, ag85C, alpha,alpha'-trehalose 6-monomycolate:alpha,alpha'-trehalose mycolyltransferase, antigen 85A, antigen 85C, cg0413, CMT1, corynomycoloyl transferase C, FbpA mycolyltransferase, mycoloyl transferase, mycolyl-transferase Ag85A, mycolyltransferase, mycolyltransferase, trehalose 6-monomycolate-trehalose, MytC
ECTree
2.3.1.122 trehalose O-mycolyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.3.1.122 - trehalose O-mycolyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 alpha,alpha-trehalose 6-monomycolate
alpha,alpha-trehalose 6,6'-dimycolate + alpha,alpha-trehalose
2 alpha,alpha-trehalose 6-mycolate
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
alpha,alpha'-trehalose 6-monomycolate + alpha,alpha'-trehalose 6-monomycolate
alpha,alpha'-trehalose 6,6'-dimycolate + alpha,alpha'-trehalose
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?
alpha,alpha-trehalose 6,6'-dimycolate + PorA
alpha,alpha-trehalose 6-monomycolate + mycoloyl-PorA
alpha,alpha-trehalose 6,6'-dimycolate + PorH
alpha,alpha-trehalose 6-monomycolate + mycoloyl-PorH
alpha,alpha-trehalose 6-mycolate
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose
alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate
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r
alpha,alpha-trehalose 6-palmitate + trehalose
alpha,alpha-trehalose + alpha,alpha-trehalose 6-palmitate
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r
alpha,alpha-trehalose monomycolate + D-glucose
D-glucose monomycolate + alpha,alpha-trehalose
2 alpha,alpha-trehalose 6-monomycolate
alpha,alpha-trehalose 6,6'-dimycolate + alpha,alpha-trehalose
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?
2 alpha,alpha-trehalose 6-monomycolate
alpha,alpha-trehalose 6,6'-dimycolate + alpha,alpha-trehalose
addition of glucose to this reaction condition results in decreased TDM production in a dose-dependent manner, which is associated with an increase in glucose monomycolate
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?
2 alpha,alpha-trehalose 6-monomycolate
alpha,alpha-trehalose 6,6'-dimycolate + alpha,alpha-trehalose
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substrate contains alpha-branched long-chain fatty acids. Only tiny amounts of trehalose dimycolate are detected. It is shown that FbpA mycolyltransferase from Mycobacterium leprae retains the ability to transfer unbranched but not alpha-branched fatty acids
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?
2 alpha,alpha-trehalose 6-mycolate
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
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-
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?
2 alpha,alpha-trehalose 6-mycolate
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
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r
alpha,alpha-trehalose 6,6'-dimycolate + PorA
alpha,alpha-trehalose 6-monomycolate + mycoloyl-PorA
O-acylation of pore-forming protein
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?
alpha,alpha-trehalose 6,6'-dimycolate + PorA
alpha,alpha-trehalose 6-monomycolate + mycoloyl-PorA
O-acylation of pore-forming protein
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?
alpha,alpha-trehalose 6,6'-dimycolate + PorH
alpha,alpha-trehalose 6-monomycolate + mycoloyl-PorH
O-acylation of pore-forming protein
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?
alpha,alpha-trehalose 6,6'-dimycolate + PorH
alpha,alpha-trehalose 6-monomycolate + mycoloyl-PorH
O-acylation of pore-forming protein
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?
alpha,alpha-trehalose 6-mycolate
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
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?
alpha,alpha-trehalose 6-mycolate
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
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?
alpha,alpha-trehalose 6-mycolate
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate
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-
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?
alpha,alpha-trehalose monomycolate + D-glucose
D-glucose monomycolate + alpha,alpha-trehalose
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Mycobacterium tuberculosis and Mycobacterium avium, replace TDM with glucose monomycolate by borrowing host-derived glucose as an alternative substrate for the FbpA mycolyltransferase
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?
alpha,alpha-trehalose monomycolate + D-glucose
D-glucose monomycolate + alpha,alpha-trehalose
trehalose dimycolate production decreases in a dose-dependent manner when glucose is added to the medium. It is hypothesized that trehalose monomycolate and glucose compete for access to the acceptor site of the Ag85A, and the enzyme preferentially catalyzes biosynthesis of glucose monomycolate, rather than trehalose dimycolate, when glucose is readily available
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?
alpha,alpha-trehalose monomycolate + D-glucose
D-glucose monomycolate + alpha,alpha-trehalose
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D-glucose is used as an alternative acceptor substrate. FbpA mycolyltransferase from Mycobacterium leprae shows only trace of glucose monomycolate is detected
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?
additional information
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enzyme additionally catalyzes the transfer of the fatty acid residue to yield O-acylated polypeptides
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additional information
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enzyme additionally catalyzes the transfer of the fatty acid residue to yield O-acylated polypeptides
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additional information
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enzyme additionally catalyzes the transfer of the fatty acid residue to yield O-acylated polypeptides
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additional information
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evaluation of the substrate tolerance of Ag85 isoforms, development of a broad Ag85 substrate, overview. Modifications on every position of the sugar scaffold are tolerated, including even C-1 methyl groups at the crowded anomeric linkages, positive charges, such as in the 2-amino-trehalose and even stereochemically incorrect 2,2'-di-fluoro-alpha,beta-mannotrehalose. Even a previously reported inhibitor 6-azido-trehalose, and putative tetrahedral intermediate analogs, such as trehalose-6-phosphate, are also processed. 2,2'-dideoxy-lyxo-trehalose is no substrate
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?
additional information
?
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the binding of the incoming acceptor molecule drives the protein to form an active conformation, the second half-reaction could therefore proceed through a direct or indirect activation pathway upon acceptor molecule binding. The beta-hydroxy group of mycolic acid plays a direct role in the second half-reaction of Ag85 catalysis
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additional information
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the binding of the incoming acceptor molecule drives the protein to form an active conformation, the second half-reaction could therefore proceed through a direct or indirect activation pathway upon acceptor molecule binding. The beta-hydroxy group of mycolic acid plays a direct role in the second half-reaction of Ag85 catalysis
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additional information
?
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the enzyme, in form of DNA and protein or peptide fragments, is immunogenic in C57BL/6 mice and effective as tuberculosis vaccine against Mycobacterium tuberculosis strain H37Rv, alone and especially in combination with PstS3 DNA and/or protein, detailed overview
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?
additional information
?
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exchange of mycolyl group, highly specific for alpha,alpha'-trehalose as the mycolate acceptor, less specific for the acyl donor group
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