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2.3.1.122: trehalose O-mycolyltransferase

This is an abbreviated version!
For detailed information about trehalose O-mycolyltransferase, go to the full flat file.

Word Map on EC 2.3.1.122

Reaction

2 alpha,alpha-trehalose 6-mycolate =

alpha,alpha-trehalose
+
alpha,alpha-trehalose 6,6'-bismycolate

Synonyms

Ag85A, ag85C, alpha,alpha'-trehalose 6-monomycolate:alpha,alpha'-trehalose mycolyltransferase, antigen 85A, antigen 85C, cg0413, CMT1, corynomycoloyl transferase C, FbpA mycolyltransferase, mycoloyl transferase, mycolyl-transferase Ag85A, mycolyltransferase, mycolyltransferase, trehalose 6-monomycolate-trehalose, MytC

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.122 trehalose O-mycolyltransferase

Purification

Purification on EC 2.3.1.122 - trehalose O-mycolyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, gel filtration and Sephadex column chromatography
-
native enzyme from strain BCG, recombinant enzyme from Escherichia coli
-
recombinant proteins purified by affinity chromatography on Ni2+ charged His-trap columns
-
using Ni-NTA chromatography
using Ni-NTA chromatography and by applying a linear imidazole gradient from 10 to 500 mM. The final purification step contains gel filtration on a Superdex 200 column
-