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2.3.1.122: trehalose O-mycolyltransferase

This is an abbreviated version!
For detailed information about trehalose O-mycolyltransferase, go to the full flat file.

Word Map on EC 2.3.1.122

Reaction

2 alpha,alpha-trehalose 6-mycolate =

alpha,alpha-trehalose
+
alpha,alpha-trehalose 6,6'-bismycolate

Synonyms

Ag85A, ag85C, alpha,alpha'-trehalose 6-monomycolate:alpha,alpha'-trehalose mycolyltransferase, antigen 85A, antigen 85C, cg0413, CMT1, corynomycoloyl transferase C, FbpA mycolyltransferase, mycoloyl transferase, mycolyl-transferase Ag85A, mycolyltransferase, mycolyltransferase, trehalose 6-monomycolate-trehalose, MytC

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.122 trehalose O-mycolyltransferase

Engineering

Engineering on EC 2.3.1.122 - trehalose O-mycolyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S189V
S130L
-
mutant S130L of Mycobacterium avium exhibits a reduced mycosyltransferase activity compared to wild-type
L130S
-
leucine at position 130 of Mycobacterium leprae FbpA limits access of mycolate-containing glycolipid substrates to their binding site. Mutant L130S exhibits an enhanced mycosyltransferase activity compared to wild-type while the ability to transfer the unbranched acyl chain is unchanged. Km (substrate: short-chain trehalose monomycolate): 0.043 mM, sharply decreased compared to wild-type
S148A
mutant lacks observable transesterase activity but exhibits hydrolase activity at a lower level than that of wild-type
S148T
mutant lacks observable transesterase activity but exhibits hydrolase activity at a lower level than that of wild-type
S148A
-
mutant lacks observable transesterase activity but exhibits hydrolase activity at a lower level than that of wild-type
-
S148T
-
mutant lacks observable transesterase activity but exhibits hydrolase activity at a lower level than that of wild-type
-
additional information
-
deletion mutant of enzyme genes cmytA and cmytB. Double mutant fails to grow at 34°C. It exhibits a growth defect and forms segmentation-defective cells at 30°C. Double mutant elaborates 60% less cell-wall-bound corynomycolates and produces less crystalline surface layer proteins than wild-type