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2.3.1.12: dihydrolipoyllysine-residue acetyltransferase

This is an abbreviated version!
For detailed information about dihydrolipoyllysine-residue acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.12

Reaction

acetyl-CoA
+
enzyme N6-(dihydrolipoyl)lysine
=
CoA
+
enzyme N6-(S-acetyldihydrolipoyl)lysine

Synonyms

acetyltransferase, lipoate, DHLTA, dihydrolipoamide acetyltransferase, dihydrolipoate acetyltransferase, dihydrolipoic transacetylase, dihydrolipoyl acetyl transferase, dihydrolipoyl acetyltransferase, dihydrolipoyl acetyltransferase component E2, dihydrolipoyl acetyltransferase E2p, dihydrolipoyl transacetylase, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLAT, E2, E2p, hE2, Lat1, lipoate acetyltransferase, lipoate transacetylase, lipoic acetyltransferase, lipoic acid acetyltransferase, lipoic transacetylase, lipoylacetyltransferase, More, myelin-proteolipid O-palmitoyltransferase, palmitoyl-CoA:myelin-proteolipid O-palmitoyltransferase, pyruvate dehydrogenase complex component E2, pyruvate dehydrogenase complex component E2p, pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase component, thioltransacetylase A, transacetylase X

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.12 dihydrolipoyllysine-residue acetyltransferase

Molecular Weight

Molecular Weight on EC 2.3.1.12 - dihydrolipoyllysine-residue acetyltransferase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1000000
-
sedimentation equilibrium centrifugation
127000
-
fusion protein of 11000 Da fragment with beta-galactosidase, SDS-PAGE
1470000
-
sedimentation equilibrium experiments in 0.83 M acetic and and 0.005 M sodium chloride
150000
-
no component of pyruvate dehydrogenase complex, sucrose density gradient centrifugation
1548000
-
native enzyme
1560000
-
fluorescence anisotropy decay experiments
1800000 - 1970000
-
calculation from sedimentation and diffusion constants, meniscus depletion method
200000
2700000
gel filtration
27500
-
24 * 27500, SDS-PAGE, light scattering experiments, quarternary structure
2800000
-
calculation from subunit composition of pyruvate dehydrogenase complex
30000
-
gel filtration in the presence of guanidinium hydrochloride
30028
x * 30028, catalytic domain, calculated from amino acid sequence
30030
x * 30030, catalytic domain, FT mass spectrometry
3100000
-
-
33100
x * 33100, about, E2, sequence calculation
36000
40000
-
24 * 40000, linked by noncovalent bonds
42000
-
60 * 42000, SDS-PAGE
45953
-
x * 45953, recombinant 1-lip E2, mass spectrometry, x * 8982, recombinant unacetylated hybrid lipoyl domain, mass spectrometry, x * 9019, recombinant fully acetylated hybrid lipoyl domain, mass spectrometry
46265
-
x * 46265, calculation from nucleotide sequence
46930
calculation from nucleotide sequence
48000
-
24 * 48000
48546
-
x * 48546, calculation from nucleotide sequence
500000
50080
-
calculation from nucleotide sequence
51000
-
gel filtration
52000
52000 - 53000
SDS-PAGE
530000
58000 - 59000
-
SDS-PAGE
58890
-
calculation from nucleotide sequence
59000
60000
60100 - 64500
-
sedimentation equilibrium, depending on buffer
63000
-
sedimentation equilibrium
64260
-
calculation from nucleotide sequence
64910
-
calculation from nucleotide sequence
65959
65960
-
calculation from nucleotide sequence
66000
-
30 * 66000, SDS-PAGE, sedimentation equilibrium
67000
-
plastid precursor protein, SDS-PAGE
670000
68000
-
SDS-PAGE
70000
73000 - 75000
-
SDS-PAGE
74000
78000
-
SDS-PAGE
80000
81000
-
SDS-PAGE, gel filtration
82000
-
3 * 82000, gel filtration, trimeric form occurs in solutions with 4 M guanidine hydrochloride
83000
864000
-
crystallization experiments
8982
-
x * 45953, recombinant 1-lip E2, mass spectrometry, x * 8982, recombinant unacetylated hybrid lipoyl domain, mass spectrometry, x * 9019, recombinant fully acetylated hybrid lipoyl domain, mass spectrometry
9019
-
x * 45953, recombinant 1-lip E2, mass spectrometry, x * 8982, recombinant unacetylated hybrid lipoyl domain, mass spectrometry, x * 9019, recombinant fully acetylated hybrid lipoyl domain, mass spectrometry
additional information