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2.3.1.118: N-hydroxyarylamine O-acetyltransferase

This is an abbreviated version!
For detailed information about N-hydroxyarylamine O-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.118

Reaction

acetyl-CoA
+
an N-hydroxyarylamine
=
CoA
+
an N-acetoxyarylamine

Synonyms

acetyltransferase, N-hydroxyarylamine O-, arylamine N-acetyltransferase, arylhydroxamate N,O-acetyltransferase, N-arylamine acetyltransferase, N-hydroxy-2-aminofluorene-O-acetyltransferase, N-hydroxyarylamine O-acetyltransferase, NhoA, NhoA1, NhoA2

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.118 N-hydroxyarylamine O-acetyltransferase

Purification

Purification on EC 2.3.1.118 - N-hydroxyarylamine O-acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, ion exchange chromatography, gel filtration, immunoaffinity chromatograpy with protein A Sepharose columns
-
copurifies and may be identical with N-acetyltransferase EC 2.3.1.5
-
copurifies with arylamine acetyltransferase and arylhydroxamic acid N,O-acetyltransferase
-
monomeric isozyme
-
Ni-NTA column chromatography
-
nickel affinity column chromatography, SDS-PAGE
-
partial, by ammonium sulfate precipitation, DEAE-cellulose and Sephadex-G150 column chromatography
-
partial, using DEAE Cellulose and Sephacryl S-200 chromatography
-
purification by ammonium sulfate followed by DEAE cellulose, gel filtration on GCL-2000 and HPKB-hydroxyapatite chromatography
-
single step immobilized metal ion chromatography
-
streptomycin, ammonium sulfate precipitation, DEAE Cellulose, Sephadex G-150 chromatograpy
-