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2.3.1.107: deacetylvindoline O-acetyltransferase

This is an abbreviated version!
For detailed information about deacetylvindoline O-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.107

Reaction

acetyl-CoA
+
deacetylvindoline
=
CoA
 +
vindoline

Synonyms

17-O-deacetylvindoline O-acetyltransferase, 17-O-deacetylvindoline-17-O-acetyltransferase, acetyl-CoA-17-O-deacetylvindoline 17-O-acetyltransferase, acetyl-CoA: 4-O-deacetylvindoline 4-O-acetyltransferase, acetylcoenzyme A-deacetylvindoline 4-O-acetyltransferase, acetylcoenzyme A:deacetylvindoline 4-O-acetyltransferase, acetylcoenzyme A:deacetylvindoline O-acetyltransferase, acetyltransferase, deacetylvindoline, DAT, deacetyl vindoline 4-O-acetyl transferase, deacetylvindoline acetyl CoA acetyltransferase, deacetylvindoline acetyl-CoA acetyl transferase, deacetylvindoline acetyltransferase, deacetylvindoline-4-O-acetyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.107 deacetylvindoline O-acetyltransferase

Cloned

Cloned on EC 2.3.1.107 - deacetylvindoline O-acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme is expressed as recombinant His-tagged protein in Escherichia coli
-
expressed in Escherichia coli and Catharanthus roseus
-
expression in Escherichia coli
gene Dat, DNA sequence determination and analysis
the CrDAT transgene is transferred into Catharanthus roseus by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The Catharanthus roseus DAT gene is introduced into Catharanthus roseus plants and it ias confirmed that CrDAT is successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein
transformation to Agrobacterium tumefaciens
transient expression of N-terminally or C-terminally GFP-tagged DAT in Catharanthus roseus cells. Irrespective to the fusion orientation, DAT is localized to the cytoplasm and the nucleus, as highlighted by the perfect overlapping of the fluorescence signal of the fusion protein with the nucleocytoplasmic CFP marker
-