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2.3.1.1: amino-acid N-acetyltransferase

This is an abbreviated version!
For detailed information about amino-acid N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.1

Reaction

acetyl-CoA
+
L-glutamate
=
CoA
 +
N-acetyl-L-glutamate

Synonyms

acetylglutamate synthase, acetylglutamate synthetase, acetylglutamic synthetase, acetyltransferase, amino acid, AGAS, amino acid acetyltransferase, ARG2, ArgA, ArgH(A), argJ, Cg3035, More, N-acetyl-glutamate synthase, N-acetyl-L-glutamate synthase, N-acetyl-L-glutamate synthase/kinase, N-acetyl-L-glutamate synthetase, N-acetylglutamate synthase, N-acetylglutamate synthase/kinase, N-acetylglutamate synthetase, NAGS, NAGS-K, NAGS/K, NAT, ngNAGS, PaNAGS, pitax, Rv2747, SINAGS1, XcNAGS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.1 amino-acid N-acetyltransferase

Cloned

Cloned on EC 2.3.1.1 - amino-acid N-acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli as a His-tagged fusion protein
cloning of N-acetylglutamate synthase gene argA and a mutant gene fbr-argA responsible for arginine feedback resistance
coexpression of N-acetylglutamate synthase gene ARG2 together with ARG5 and ARG6 coding for acetylglutamate kinase and acetylglutamyl-phosphate reductase in Saccharomyces cerevisiae
-
expressed in an Escherichia coli JM109 DELTAargA mutant
CAF20762, Q8NM40
expressed in Escherichia coli BL21 AI cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) cells
-
expression in Escherichia coli
expression in Escherichia coli BL21
gene cg3035, genomic organisation, gene cg3035 has an operon structure together with gene cg3034, expression of gene cg3035 complements an argA-deficient Escherichia coli mutant JM109 strain, recombinant overexpression in Corynebacterium glutamicum strain ATTC 13032
CAF20762, Q8NM40
gene PA1377, cloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21(DE3)
gene Rv2747 or argA, expression of the His-tagged enzyme in Escherichia coli
-
gene structure, His-tagged wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain NK 5992
-
mutent enzymes from patients with NAGS missense mutations are overexpressed in Escherichia coli NK5992. All mutated proteins show severe decrease in enzyme activity providing evidence for the disease-causing nature of the mutations
-
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain argA-
-
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain argA-, overexpression of the preenzyme in insect cells via baculovirus infection system, 2 processed forms with different N-terminal truncations are produced
-
phylogenetic analysis
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged wild-type and mutant enzymes, and isolated catalytic N-acetyltransferase domain in Escherichia coli strain BL21(DE3)
transfection to Arabidopsis thaliana