2.2.1.10: 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase

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For detailed information about 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase, go to the full flat file.

Reaction

Synonyms

ADHS, 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, ADH synthase, ADHS, MJ0400, More

ECTree

     2 Transferases

         2.2 Transferring aldehyde or ketonic groups

             2.2.1 Transketolases and transaldolases

                2.2.1.10 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase

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Results	in table
1942	AA Sequence
3	Cloned(Commentary)
1	Crystallization (Commentary)
4	General Information
2	Inhibitors
1	Ki Value [mM]
1	KM Value [mM]
3	Natural Substrates/ Products (Substrates)
3	Organism
6	Pathway
2	pH Optimum
3	Purification (Commentary)
2	Reaction
3	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
7	Substrates and Products (Substrate)
2	Subunits
9	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]

Purification

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Purification on EC 2.2.1.10 - 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

recombinant enzyme from Escherichia coli by heat treatment and anion exchange chromatography

Methanocaldococcus jannaschii

Q57843

718838

recombinant His-tagged ADHS from Escherichia coli strain B834-(DE3) by nickel affinity chromatography, cleavage of the tag by thrombin, and further by strepavidin affinity chromatography to eliminate thrombin, followed by gel filtration/ultrafiltration

Methanocaldococcus jannaschii

Q57843

718845

soluble fraction of recombinant His6-tagged MJ0400 from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography to over 97% purity

Methanocaldococcus jannaschii

Q57843

692386