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2.2.1.1: transketolase

This is an abbreviated version!
For detailed information about transketolase, go to the full flat file.

Word Map on EC 2.2.1.1

Reaction

sedoheptulose 7-phosphate
+
D-glyceraldehyde 3-phosphate
=
D-ribose 5-phosphate
 +
D-xylulose 5-phosphate

Synonyms

glycolaldehydetransferase, STM14_2885, STM14_2886, TK16, TKA, TKL, TKL1, Tkl2, TKT, TKT10, TKT3, TKT7, TktA, TktB, TKTc, TKTL-1, TKTL1, TKTL2, TKTp, transketolase, transketolase 10, transketolase 3, transketolase 7, transketolase A, transketolase B, transketolase like 1, transketolase-1, transketolase-like 1, transketolase-like enzyme 1, transketolase-like-1, transketolase-like-1-gene, transketolase-like-2

ECTree

     2 Transferases
         2.2 Transferring aldehyde or ketonic groups
             2.2.1 Transketolases and transaldolases
                2.2.1.1 transketolase

Purification

Purification on EC 2.2.1.1 - transketolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Ni-NTA column chromatography and Superdex S200 gel filtration
-
His-tagged wild-type transketolase purified on Ni-NTA resin
immunoaffinity chromatography
Ni-NTA column chromatography
Ni2+-NTA agarose resin column chromatography and Superdex S-200 gel filtration
nickel-chelation column chromatography and gel filtration
no isozymes, inseparable from triosephosphate isomerase EC 5.3.1.1
-
on Ni-NTA resin
-
on Ni-NTA resin-bound MarR
-
on Ni2+-NTA resin and by ammonium sulfate precipitation
-
partial
-
partial, 5 multiple forms by isoelectric focusing
-
recombinant enzyme
-
recombinant protein
to homogeneity
-
wild-type and mutant A29E purified on Ni-NTA resin
-
wild-type and mutant D469T
-
YEA-His Spin column chromatography
-