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2.1.3.9: N-acetylornithine carbamoyltransferase

This is an abbreviated version!
For detailed information about N-acetylornithine carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.9

Reaction

Carbamoyl phosphate
+
N2-Acetyl-L-ornithine
=
phosphate
+
N-acetyl-L-citrulline

Synonyms

acetylornithine transcarbamylase, AOTC, AOTCase, MJ0366, More, N-acetyl-L-ornithine transcarbamylase, N-acetylornithine carbamoyltransferase, N-acetylornithine transcarbamoylase, N-acetylornithine transcarbamylase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.9 N-acetylornithine carbamoyltransferase

Crystallization

Crystallization on EC 2.1.3.9 - N-acetylornithine carbamoyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure in presence and absence of N-acetylcitrulline
-
purified recombinant enzyme in binary complex with its substrates, carbamoyl phosphate or N-acetyl-L-ornithine, and in ternary complex with carbamoyl phosphate and N-acetyl-L-norvaline, hanging drop vapour diffusion method, preparation by mixing of 0.002 ml of 12 mg/ml protein solution with an equal volume of reservoir solution containing the ligands, cryoprotection, X-ray diffraction structure determination and analysis at 1.8-1.95 A resolution, modeling of the transition state complex
-
wild-type and mutant AOTCase complexed with bisubstrate analogue Ndelta-(phosphonoacetyl)-Nalpha-acetyl-L-ornithine, hanging drop vapor diffusion method, mixing of 0.002 ml 10 mg/ml protein in solution with 0.0016 ml of reservoir solution and 0.0004 ml 10 mM ligand solution. The reservoir solution contains 20% w/v PEG 3350, 0.2 M lithium sulfate, and 0.1 M Bis-Tris, pH 6.0, X-ray diffraction structure determination and analysis at 1.8-2.2 A resolution, molecular replacement