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2.1.3.6: putrescine carbamoyltransferase

This is an abbreviated version!
For detailed information about putrescine carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.6

Reaction

Carbamoyl phosphate
+
putrescine
=
phosphate
 +
N-Carbamoylputrescine

Synonyms

anabolic putrescine carbamoyltransferase, aPTC, catabolic putrescine carbamoyltransferase, cPTC, Lmo0036, PTC, PTCase, putrescine carbamoyltransferase, putrescine synthase, putrescine transcarbamylase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.6 putrescine carbamoyltransferase

Purification

Purification on EC 2.1.3.6 - putrescine carbamoyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
65°C, ammonium sulfate, DEAE-Sephadex, Sephadex G-200
-
ammonium sulfate, heat treatment, Sephadex G-100
-
MnCl2, ammonium sulfate, putrescine-CH-Sepharose affinity column
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged protein from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration