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2.1.3.3: ornithine carbamoyltransferase

This is an abbreviated version!
For detailed information about ornithine carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.3

Reaction

Carbamoyl phosphate
+
L-ornithine
=
phosphate
+
L-citrulline

Synonyms

anabolic ornithine carbamoyltransferase, anabolic ornithine transcarbamoylase, AOTC, arcB gene product, ArgF, ArgK, aTtOTCase, carbamoyltransferase, carbamoyltransferase, ornithine, carbamylphosphate-ornithine transcarbamylase, catabolic ornithine carbamoyltransferase, catabolic ornithine transcarbamylase, citrulline phosphorylase, cOTC, L-ornithine carbamoyltransferase, L-ornithine carbamyltransferase, L-ornithine transcarbamylase, Lmo0036, MB1684, Mtb OTC, OCT, ornithine carbamoyltransferase, ornithine carbamoytransferase, ornithine carbamyl transferase, ornithine carbamyltransferase, ornithine transcarbamoylase, ornithine transcarbamylase, ornithine transcarbamylase 3, OTC, otcase, ROTCase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.3 ornithine carbamoyltransferase

Purification

Purification on EC 2.1.3.3 - ornithine carbamoyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
60-65°C for 5 min, ammonium sulfate, DEAE-cellulose, Bio-Gel P-300
-
affinity chromatography on delta-N-(phosphonacetyl)-L-ornithine, Sephacryl S-200
-
ammonium sulfate
-
ammonium sulfate, 70°C for 25 min, gel filtration, ion-exchange chromatography
-
ammonium sulfate, DEAE-cellulose, aminohexyl-agarose
-
ammonium sulfate, DEAE-cellulose, isoforms 1 and 2
-
ammonium sulfate, DEAE-Sephadex, Sephadex G-200
-
ammonium sulfate, DEAE-Sepharose, ornithine[AcPO(OH)2]-Sepharose
-
ammonium sulfate, heat treatment, celite, DEAE-Sephadex A-50, Sephacryl S-200, partial purification
-
ammonium sulfate, heat, activated charcoal, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography
-
ammonium sulfate, Sephacryl S-200, delta-N-phosphonoacetyl-L-ornithine affinity chromatography
-
ammonium sulfate, streptomycin, DEAE-cellulose, heating, DEAE-Sephadex
-
DEAE-cellulose, isoelectric focusing
-
DEAE-Sephacel, hydroxylapatite, preparative PAGE, isoenzyme 1
-
DEAE-Sephadex, hydoxyapatite C, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography
-
delta-N-(phosphonoacetyl)-L-ornithine-Sepharose 6B affinity chromatography
-
enzyme from normal liver and liver of a Reye's syndrome patient, DEAE-cellulose, hydroxylapatite, Sephadex G-200
-
gel filtration, ammonium sulfate-mediated hydrophobic chromatography
gene argF, expression of His-tagged aOTC in Escherichia coli strain BL21-CodonPlus(DE3)-RIL
heat treatment, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography
-
heat, ammonium sulfate, DEAE-cellulose, hydroxylapatite, DEAE-Sephacel, Bio-Gel A 1.5
-
heat, ammonium sulfate, DEAE-Sephadex, Sephadex G-200, aminohexyl-Sepharose
-
high-performance hydrophobic interaction chromatography
-
HisTrap column chromatography and Superdex 200 gel filtration
immunoadsorbent column
-
isoforms 1 and 2
-
N-delta-(phosphonoacetyl)ornithine affinity chromatography, Sephadex G-100
-
native enzyme by affinity chromatography using delta-N-(phosphonacetyl)-L-ornithine
-
native enzyme from liver mitochondria by anion exchange chromatography, ultrafiltration, and gel filtration, to about 50% purity
-
Ni-NTA column chromatography
-
partially purified
-
plasmid-containing enzyme-overproducing strain
-
protamine sulfate, ammonium sulfate, diethylaminoethyl-cellulose, Sephadex G-150, hydroxylapatite
-
putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase, ammonium sulfate, putrescine-Sepharose affinity column
-
recombinant enzyme
recombinant enzyme, Mono Q, arginine-Sepharose column
-
recombinant enzyme, thermodenaturation at 85°C, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography
-
recombinant His-tagged enzyme
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography
recombinant protein using His-tag
-
recombinant wild-type and R277W mutant enzyme, ammonium sulfate, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography
-
single step procedure using delta-N-(phosphonoacetyl)-L-ornithine-Sepharose 6B affinity chromatography
-