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2.1.3.3: ornithine carbamoyltransferase

This is an abbreviated version!
For detailed information about ornithine carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.3

Reaction

Carbamoyl phosphate
+
L-ornithine
=
phosphate
 +
L-citrulline

Synonyms

anabolic ornithine carbamoyltransferase, anabolic ornithine transcarbamoylase, AOTC, arcB gene product, ArgF, ArgK, aTtOTCase, carbamoyltransferase, carbamoyltransferase, ornithine, carbamylphosphate-ornithine transcarbamylase, catabolic ornithine carbamoyltransferase, catabolic ornithine transcarbamylase, citrulline phosphorylase, cOTC, L-ornithine carbamoyltransferase, L-ornithine carbamyltransferase, L-ornithine transcarbamylase, Lmo0036, MB1684, Mtb OTC, OCT, ornithine carbamoyltransferase, ornithine carbamoytransferase, ornithine carbamyl transferase, ornithine carbamyltransferase, ornithine transcarbamoylase, ornithine transcarbamylase, ornithine transcarbamylase 3, OTC, otcase, ROTCase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.3 ornithine carbamoyltransferase

General Stability

General Stability on EC 2.1.3.3 - ornithine carbamoyltransferase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
1 mM carbamoyl phosphate or 20% glycerol stabilize the enzyme in cell-free extracts, a minimal enzyme concentration of 0.2 mg/ml is obligatory for stability
-
cetyltrimethylammonium stabilizes enzyme in crude extract against heat inactivation in presence of the detergent
-
glycerol, 20% v/v, stabilizes during freeze-thawing
-
in Saccharomyces cerevisiae extracts the enzyme remains as stable as in Pyrococcus furiosus extracts
in the presence of trehalose the enzyme is stabilized and it also inhibits the combined denaturation effect induced by urea and high temperature.Trehalose avoids the denaturation process through a process preserving the tertiary and secondary structures and it decreases the exposure of SH-group essential for enzymatic activity maintenance
-
loss of activity after the second thawing
-
loss of activity by freezing and thawing
-
ornithine carbamoyltransferase unfolding states in the presence of urea and guanidine hydrochloride at 37°C, overview
-
prolonged dialysis and freeze-thawing causes loss of activity
-
purified enzyme is highly unstable even in the presence of glycerol, dithiothreitol and Mg2+
-
unstable below 1 M KCl