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2.1.3.3: ornithine carbamoyltransferase

This is an abbreviated version!
For detailed information about ornithine carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.3

Reaction

Carbamoyl phosphate
+
L-ornithine
=
phosphate
 +
L-citrulline

Synonyms

anabolic ornithine carbamoyltransferase, anabolic ornithine transcarbamoylase, AOTC, arcB gene product, ArgF, ArgK, aTtOTCase, carbamoyltransferase, carbamoyltransferase, ornithine, carbamylphosphate-ornithine transcarbamylase, catabolic ornithine carbamoyltransferase, catabolic ornithine transcarbamylase, citrulline phosphorylase, cOTC, L-ornithine carbamoyltransferase, L-ornithine carbamyltransferase, L-ornithine transcarbamylase, Lmo0036, MB1684, Mtb OTC, OCT, ornithine carbamoyltransferase, ornithine carbamoytransferase, ornithine carbamyl transferase, ornithine carbamyltransferase, ornithine transcarbamoylase, ornithine transcarbamylase, ornithine transcarbamylase 3, OTC, otcase, ROTCase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.3 ornithine carbamoyltransferase

Engineering

Engineering on EC 2.1.3.3 - ornithine carbamoyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H121R
-
isoform 2, 80% increase in ornithine dependent activity, 64% decrease in canaline-dependent activity
L118M
R54G
site-directed mutagenesis, inactive mutant, not exhibiting any PTC or OTC activity
Y230V/G231S/L232M/Y233G
engineering of the 230-loop of the enzyme, by replacing the sequence 230YGLY233 of the putrescine signature by its OTC counterpart VSMG, favors the use of ornithine and impairs that of putrescine
C273A
the mutant shows 2.8 and 3.2fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
D140N
the mutant shows 5.4 and 28fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
D231A
the mutant shows 450 and 580fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
E299D
the mutant shows 2.5 and 4.2fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
E299Q
the mutant shows 110 and 51fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
H272L
the mutant shows 310 and 120fold and decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
H272N
the mutant shows 4.2 and 3.4fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
Q104L
the mutant shows 5.9 and 10fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
Q106E
-
mutant to study the carbamoyl phosphate cooperativity on the anabolic OTCase
R57A
the mutant shows 57 and 44fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
S61A
the mutant shows 6.1 and 1.8fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
Y160F
the mutant shows 6.7 and 8.2fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
Y160S
the mutant shows 1.5 and 14fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
Y229F
the mutant shows 3.5 and 2.7fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
Y229S
the mutant shows 1.7 and 1.4fold decrease of activity with respect to carbamoyl phosphate and L-ornithine, respectively, compared to the wild type enzyme
K88Q
-
less than 1% of wild-type activity
K88R
-
mutant retains substantial enzymatic activity
R277W
-
shows no substrate inhibition by ornithine, 70fold lower affinity for L-ornithine
E105G
E106A
-
mutant arcB6240, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity
E106G
-
mutant arcB6254, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity
E106A
-
mutant arcB6240, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity
-
E106G
-
mutant arcB6254, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity
-
A240D
-
10fold increase of Km at 55°C
E277G
-
14fold increase of Km at 55°C
Y227D
-
slight increase of Km at 55°C
D182N
-
greatly reduced affinity for L-ornithine, no interaction with arginase
E123A
-
little change in activity, or in sensitivity to arginase
E123S
-
little change in activity, or in sensitivity to arginase
E256A
-
greatly reduced affinity for L-ornithine, impaired interaction with arginase
E256Q
-
greatly reduced affinity for L-ornithine, impaired interaction with arginase
G181R
-
no residual activity
K260A
-
important in mediating sensitivity to L-ornithine and arginase
K260R
-
important in mediating sensitivity to L-ornithine and arginase
K263R
-
important in mediating sensitivity to L-ornithine and arginase
K265A
-
important in mediating sensitivity to L-ornithine and arginase
K265R
-
important in mediating sensitivity to L-ornithine and arginase
K268A
-
important in mediating sensitivity to L-ornithine and arginase
K268R
-
important in mediating sensitivity to L-ornithine and arginase
K289S
-
greatly reduced affinity for L-ornithine, impaired interaction with arginase
L290Q
-
reduced activity, little change in sensitivity to arginase
L290S
-
reduced activity, little change in sensitivity to arginase
N184Q
-
greatly reduced affinity for L-ornithine, impaired interaction with arginase
N185Q
-
greatly reduced affinity for L-ornithine, impaired interaction with arginase
Q294P
-
little change in activity, or in sensitivity to arginase
T68G
-
greatly reduced activity, no change in sensitivity to arginase
additional information