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2.1.3.1: methylmalonyl-CoA carboxytransferase

This is an abbreviated version!
For detailed information about methylmalonyl-CoA carboxytransferase, go to the full flat file.

Word Map on EC 2.1.3.1

Reaction

(S)-methylmalonyl-CoA
+
pyruvate
=
propanoyl-CoA
 +
oxaloacetate

Synonyms

5S subunit of transcarboxylase, carboxyltransferase subunit of acetyl-CoA carboxylase, carboxyltransferase, methylmalonyl coenzyme A, DtsR1, MCT, methyl malonyl CoA carboxyl transferase, methylmalonyl CoA carboxyltransferase, methylmalonyl CoA-oxalacetate transcarboxylase, methylmalonyl coenzyme A carboxyltransferase, methylmalonyl-CoA carboxyltransferase, methylmalonyl-CoA transcarboxylase, MMC, oxalacetic transcarboxylase, transcarboxylase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.1 methylmalonyl-CoA carboxytransferase

Purification

Purification on EC 2.1.3.1 - methylmalonyl-CoA carboxytransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a deletion mutant and recombinant enzyme
-
affinity chromatography on avidin-Sepharose
on chitin column beads, the intein tag is removed by auto-splicing
-
radioactive labeling of Propionibacterium freudenreichii proteins is performed, analysis of the proteins by two-dimensional gel electrophoresis follows
-
recombinant 5S subunit, single step purification using Intein mediated protein ligation method and cleavage from beads by dithiothreitol
-
recombinant enzyme and mutant 1.3S subunit
-
recombinant enzyme and mutant 1.3S subunit, affinity chromatography on avidin(monomeric)-agarose, copurification of apo and biotinylated 1.3S forms
-
separation of transcarboxylase complexes from uncombined 12S, 5S and 1.3S subunits by gel filtration
-
the expressed 5S subunit is purified to apparent homogeneity by a single step process by using Intein mediated protein ligation method
-
using a chelating Sepharose FF column with immobilized Ni2+
-