2.1.3.1: methylmalonyl-CoA carboxytransferase

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For detailed information about methylmalonyl-CoA carboxytransferase, go to the full flat file.

Reaction

Synonyms

5S subunit of transcarboxylase, carboxyltransferase subunit of acetyl-CoA carboxylase, carboxyltransferase, methylmalonyl coenzyme A, DtsR1, MCT, methyl malonyl CoA carboxyl transferase, methylmalonyl CoA carboxyltransferase, methylmalonyl CoA-oxalacetate transcarboxylase, methylmalonyl coenzyme A carboxyltransferase, methylmalonyl-CoA carboxyltransferase, methylmalonyl-CoA transcarboxylase, MMC, oxalacetic transcarboxylase, transcarboxylase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.3 Carboxy- and carbamoyltransferases

                2.1.3.1 methylmalonyl-CoA carboxytransferase

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Results	in table
2155	AA Sequence
5	Application
1	CAS Registry Number
16	Cloned(Commentary)
22	Cofactor
6	Crystallization (Commentary)
6	General Information
5	General Stability
18	Inhibitors
9	Ki Value [mM]
12	KM Value [mM]
5	Localization
20	Metals/Ions
26	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
41	Organism
1	Oxidation Stability
5	Pathway
31	PDB ID
1	pH Optimum
1	pH Range
7	pH Stability
6	pI Value
3	Posttranslational Modification
12	Protein Variants
18	Purification (Commentary)
13	Reaction
5	Reaction Type
36	Reference
2	Source Tissue
19	Specific Activity [micromol/min/mg]
2	Storage Stability
49	Substrates and Products (Substrate)
25	Subunits
27	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
3	Temperature Stability [°C]
1	Turnover Number [1/s]

Cloned

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Cloned on EC 2.1.3.1 - methylmalonyl-CoA carboxytransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

1.3S subunit cloned and expressed in Escherichia coli

Propionibacterium freudenreichii subsp. shermanii

-

485822, 485825, 485827, 485828, 485829, 485832

12S subunit cloned and expressed in Escherichia coli

Propionibacterium freudenreichii subsp. shermanii

-

485830

5S subunit cloned and expressed in Escherichia coli

Propionibacterium freudenreichii subsp. shermanii

-

485831, 485832

5S subunit of enzyme

Propionibacterium freudenreichii subsp. shermanii

-

676760

a prokaryotic expression system for the production of a 13C- and 15N-labeled 1.3S subunit mutant that is suitable for structure determination and functional studies by NMR

Propionibacterium freudenreichii subsp. shermanii

-

485834

expressed in Escherichia coli strain SBF15

Propionibacterium freudenreichii subsp. shermanii

-

756213

gene mmc, co-overexpression of pyruvate carboxylase, methylmalonyl-CoA decarboxylase, and methylmalonyl-CoA carboxyltransferase from Propionibacterium acidipropionici ATCC 4875 in Propionibacterium shermanii, the MMC activity increases by 107%

Propionibacterium freudenreichii

-

737181

the 5S subunit protein is expressed as intein fusion protein in pTXB1 vector at the N-terminus in BL21DE3 Escherichia coli cells

Propionibacterium freudenreichii subsp. shermanii

-

689897

the dtsR1 gene is subcloned from a pUC19 plasmid carrying the dtsR1 gene into the pET26b+ expression vector

Corynebacterium glutamicum

-

684167

the gene encoding the 5S subunit is cloned into the pTXB1 vector

Propionibacterium freudenreichii subsp. shermanii

-

676760