2.1.2.9: methionyl-tRNA formyltransferase

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For detailed information about methionyl-tRNA formyltransferase, go to the full flat file.

Reaction

Synonyms

FMT, FMT1, Fmt1p, formyl-methionyl transferase, formylmethionyl-transfer ribonucleic synthetase, methionyl ribonucleic formyltransferase, methionyl-transfer ribonucleic transformylase, methionyl-transfer RNA transformylase, methionyl-tRNA formyltransferase, methionyl-tRNA Met formyltransferase, methionyl-tRNA transformylase, methionyl-tRNA-Formyltransferase, methyltransferase, methionyl-transfer ribonucleate, mitochondrial methionyl-tRNA formyltransferase, MTF, MTFMT, N10-formyltetrahydrofolic-methionyl-transfer ribonucleic transformylase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.2 Hydroxymethyl-, formyl- and related transferases

                2.1.2.9 methionyl-tRNA formyltransferase

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2	Activating Compound
46946	AA Sequence
1	CAS Registry Number
14	Cloned(Commentary)
29	Cofactor
7	Crystallization (Commentary)
77	Disease/ Diagnostics
18	General Information
3	General Stability
17	Inhibitors
5	Ki Value [mM]
57	KM Value [mM]
27	Localization
27	Metals/Ions
11	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
337	Organism
3	Pathway
18	PDB ID
5	pH Optimum
3	pH Range
71	Protein Variants
18	Purification (Commentary)
1	Reaction
1	Reaction Type
58	Reference
5	Source Tissue
12	Specific Activity [micromol/min/mg]
8	Storage Stability
239	Substrates and Products (Substrate)
5	Subunits
33	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
32	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.1.2.9 - methionyl-tRNA formyltransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-15°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, several months, stable

Escherichia coli

-

485795

-20°C, 1 month, 30% loss of activity

Escherichia coli

-

485794

-20°C, 20 mM imidazole, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, stable

Escherichia coli

-

485811

-20°C, 20 mM imidazole, pH 7.5, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, 2-4 months, stable

Escherichia coli

-

485811

-20°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, 6 months, stable

Escherichia coli

-

485797

-20°C, longer than 4 months, stable

Triticum aestivum

-

485804

25°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 150 mM KCl, 2 days, stable

Escherichia coli

-

485795, 485797