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2.1.2.9: methionyl-tRNA formyltransferase

This is an abbreviated version!
For detailed information about methionyl-tRNA formyltransferase, go to the full flat file.

Word Map on EC 2.1.2.9

Reaction

10-formyltetrahydrofolate
+
L-methionyl-tRNAfMet
=
tetrahydrofolate
 +
N-formylmethionyl-tRNAfMet

Synonyms

FMT, FMT1, Fmt1p, formyl-methionyl transferase, formylmethionyl-transfer ribonucleic synthetase, methionyl ribonucleic formyltransferase, methionyl-transfer ribonucleic transformylase, methionyl-transfer RNA transformylase, methionyl-tRNA formyltransferase, methionyl-tRNA Met formyltransferase, methionyl-tRNA transformylase, methionyl-tRNA-Formyltransferase, methyltransferase, methionyl-transfer ribonucleate, mitochondrial methionyl-tRNA formyltransferase, MTF, MTFMT, N10-formyltetrahydrofolic-methionyl-transfer ribonucleic transformylase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.9 methionyl-tRNA formyltransferase

Engineering

Engineering on EC 2.1.2.9 - methionyl-tRNA formyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A172L
site-directed mutagenesis, the mutation causes a 6fold decrease in enzyme activity compared to wild-type, the Vmax/Km of the mutant is about 4fold lower than that of the wild-type enzyme
A172S
site-directed mutagenesis, the mutation does not significantly affect the enzyme activity
A55T
-
no suppressor mutation
A89L
site-directed mutagenesis, the Vmax/Km of the mutant is about 144fold lower than that of the wild-type enzyme
A89S
site-directed mutagenesis, the mutation does not significantly affect the enzyme activity
G178D
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E
G178E
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors
G178K
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
G178L
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
G41Q
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
R38K
-
mutant enzyme with 2.2fold increased Km, no effect on kcat
R38L
-
mutant enzyme with up to 12fold lower specific activity, 6fold increased Km and 5fold decreased kcat
R42K
-
mutant enzyme with lower activity, 16fold increased Km
R42L
-
single mutant and G41R/R42L double mutant with low activity, R42L: 17fold increased Km and 79fold decreased kcat
R42Q
-
mutant enzyme with lower activity, 16-17fold increased Km
A55T
-
no suppressor mutation
-
G178D
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G178E
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors
-
G178K
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G178L
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G41D
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41K
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
G41L
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41Q
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41R
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
G41D
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41K
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
G41L
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41Q
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41R
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
A55T
-
no suppressor mutation
-
G178D
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, it compensates for lysine that is attached to the tRNA, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G178E
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors
-
G178K
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G178L
-
suppressor mutation, compensates for the formylation defect of a mutant elongator lysine-tRNAmMet, G178D, G178K and G178L are progressively weaker suppressors than G178E
-
G41D
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41K
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
G41L
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41Q
-
no suppressor mutation, mutant is stably overproduced in Escherichia coli CA274
-
G41R
-
suppressor mutation within a 16-amino acid insertion, compensates for the formylation defect of a U35A36/G72G73 mutant initiator tRNA, lacking a critical determinant in the acceptor stem
-
D145A
-
mutant enzyme with drastically reduced activity
D145E
-
mutant enzyme with 881fold reduced formylation rate, kinetic parameters
D145N
-
mutant enzyme with 4031fold reduced formylation rate, kinetic parameters
H109A
-
mutant enzyme with 4677fold reduced formylation rate, kinetic parameters
H109K
-
inactive mutant enzyme
H109R
-
mutant enzyme with 7172fold reduced formylation rate, kinetic parameters
K37A
-
mutant enzyme with 3.5fold reduced formylation rate, kinetic parameters
N107A
-
mutant enzyme with 48fold reduced formylation rate, kinetic parameters
R41A
-
mutant enzyme with 470fold reduced formylation rate, kinetic parameters
R41K
-
mutant enzyme with 140fold reduced formylation rate, kinetic parameters
V31A
-
mutant enzyme with 1.7fold reduced formylation rate
A59V
-
the mutation causing substantially decreased enzyme activity is associated with selective vulnerability of the visual pathway and a mild neurological phenotype of Leigh syndrome. The missense mutation destabilizes the protein
R181S
-
the mutation causes severe Leigh syndrome
S125L
naturally occurring mutation, the mutant has a 653fold lower activity compared to the wild-type
S209L
naturally occurring mutation, the mutant has a 36fold lower activity compared to the wild-type
del833
-
inactivated methionyl-tRNA formyltransferase
deltafmt1
-
in combination with mutation of AEP3, a gene encodes for a peripheral mitochondrial inner membrane protein, respiratory defect
additional information