2.1.2.8: deoxycytidylate 5-hydroxymethyltransferase
This is an abbreviated version!
For detailed information about deoxycytidylate 5-hydroxymethyltransferase, go to the full flat file.
Word Map on EC 2.1.2.8
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2.1.2.8
-
bacteriophage
-
phage
-
thymidylate
-
amber
-
deoxyribonucleotide
-
triphosphate
-
dtmp
-
dihydrofolate
-
phage-coded
-
cytosine
-
ribonucleotide
-
fork
-
t4-infected
-
dctpase
- 2.1.2.8
- bacteriophage
- phage
- thymidylate
-
amber
- deoxyribonucleotide
- triphosphate
- dtmp
- dihydrofolate
-
phage-coded
- cytosine
- ribonucleotide
-
fork
-
t4-infected
- dctpase
Reaction
Synonyms
d-cytidine 5'-monophosphate hydroxymethylase, DCH, dCMP Hmase, dCMP hydroxymethylase, deoxyCMP hydroxymethylase, deoxycytidylate hydroxymethylase, deoxycytidylate hydroxymethyltransferase, deoxycytidylic hydroxymethylase, hydroxymethyltransferase, deoxycytidylate
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Substrates Products
Substrates Products on EC 2.1.2.8 - deoxycytidylate 5-hydroxymethyltransferase
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REACTION DIAGRAM
5,10-methylenetetrahydrofolate + H2O + dCMP
tetrahydrofolate + 5-hydroxymethyl-dCMP
Tequatrovirus T4
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
bacteriophage T6r+
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
bacteriophage T6r+
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
the hydroxymethylation is catalyzed by dCMP hydroxymethylase, which transfers a methylene group from methylenetetrahydrofolate to C5 of dCMP and then uses a water molecule to hydrate the methylene group to form hydroxymethyl-dCMP, one precursor for phage DNA replication
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
the enzyme uses the solvent water as the hydroxyl group donor
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
enzyme is responsible for production of 5-hydroxymethyl-dCMP found in the DNA of the phage
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
enzyme forms part of phage DNA protection system
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
5-hydroxymethyl-dCMP is a virus-specific DNA precursor
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
-
involved in biosynthesis of DNA precursor
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
involved in biosynthesis of DNA precursor
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
stoichiometry of dCMP binding to the dimeric enzyme is 1:1 and depends upon a reduced thiol group, one thiol-containing active site per mol of dimeric enzyme, sequential order of substrate binding
-
r
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
Cys-148 is catalytically essential
-
r
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
Cys-148 is catalytically essential
-
r
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
role of active site Glu-60 is to accelerate bond cleavage between N5 of tetrahydrofolate and the methylene being transferred
-
r
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
enzyme structure, dCMP binding site, folate cofactor binding site, way of substrate binding in the active site, key catalytic residue Cys-148 acts as nucleophile to attack the C6-atom of the cytosine base
-
?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
Tequatrovirus T4
-
dCTP depresses the synthesis of 5-hydroxymethyldeoxycytidylate
-
?
?
-
bacteriophage T6r+
-
biosynthesis of enzyme de novo after infection
-
-
?
additional information
?
-
-
direct interaction between NDP kinase and dCMP hydroxymethylase
-
-
?
additional information
?
-
-
5-hydroxymethylcytosine in phage is formed by hydroxymethylation of the cytosine base in deoxycytidylate (dCMP) by deoxycytidylate hydroxymethylase, which uses the solvent water as the hydroxyl group donor
-
-
?
additional information
?
-
Tequatrovirus T4
-
enzyme also catalyzes 18O exchange from solvent water into 5-hydroxymethyl-dCMP in the absence of exogenous tetrahydrofolate and in the presence of tetrahydrofolate analogues that lack N-5, mechanism
-
-
?
additional information
?
-
Tequatrovirus T4
-
enzyme is part of the dNTP synthetase complex
-
-
?
additional information
?
-
Tequatrovirus T4
enzyme functions in vivo as a component of a multienzyme complex called dNTP synthetase
-
-
?
additional information
?
-
Tequatrovirus T4
-
enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus
-
-
?
additional information
?
-
Tequatrovirus T4
enzyme is part of a complex efficiently channeling DNA precursors to the replication apparatus
-
-
?
additional information
?
-
Tequatrovirus T4
-
enzyme is part of a complex catalyzing DNA precursor biosynthesis
-
-
?