2.1.2.11: 3-methyl-2-oxobutanoate hydroxymethyltransferase

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For detailed information about 3-methyl-2-oxobutanoate hydroxymethyltransferase, go to the full flat file.

Reaction

Synonyms

5,10-methylene tetrahydrofolate:alpha-ketoisovalerate hydroxymethyltransferase, alpha-ketoisovalerate hydroxymethyltransferase, dehydropantoate hydroxymethyltransferase, hydroxymethyltransferase, ketopantoate, ketopantoate hydroxymethyl transferase, ketopantoate hydroxymethyltransferase, KHMT, KPHMT, MOHMT, oxopantoate hydroxymethyltransferase, PanB

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.2 Hydroxymethyl-, formyl- and related transferases

                2.1.2.11 3-methyl-2-oxobutanoate hydroxymethyltransferase

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Results	in table
38557	AA Sequence
2	Application
1	CAS Registry Number
10	Cloned(Commentary)
30	Cofactor
2	Crystallization (Commentary)
10	General Information
2	General Stability
17	Inhibitors
22	KM Value [mM]
4	Localization
21	Metals/Ions
19	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
58	Organism
7	Pathway
45	PDB ID
7	pH Optimum
3	pH Range
4	pH Stability
1	Posttranslational Modification
6	Protein Variants
7	Purification (Commentary)
10	Reaction
1	Reaction Type
24	Reference
13	Specific Activity [micromol/min/mg]
1	Storage Stability
61	Substrates and Products (Substrate)
7	Subunits
26	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
2	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 2.1.2.11 - 3-methyl-2-oxobutanoate hydroxymethyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

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Escherichia coli

-

485700

19.3fold purification of recombinant enzyme 50fold overexpressed in E. coli panB mutant Hfr3000 YA139/pCEJ01

Escherichia coli

-

485707

affinity purification of recombinant PanB

Aspergillus nidulans

Q9Y7B6

485708

purification of recombinant enzyme expressed in Escherichia coli BL21 (DE3)

Mycobacterium tuberculosis

-

485710

purification of recombinant enzyme overexpressed in Escherichia coli BL21

Mycobacterium tuberculosis variant bovis

-

485706

strain K12, 2450fold purification

Escherichia coli

-

485699, 485701