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2.1.1.82: 3-methylquercetin 7-O-methyltransferase

This is an abbreviated version!
For detailed information about 3-methylquercetin 7-O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.82

Reaction

S-adenosyl-L-methionine
+
5,7,3',4'-tetrahydroxy-3-methoxyflavone
=
S-adenosyl-L-homocysteine
 +
5,3',4'-trihydroxy-3,7-dimethoxyflavone

Synonyms

7-O-methyltransferase, 7-OMT, flavone 7-O-methyltransferase, flavonoid O-methyltransferase, flavonol 7-O-methyltransferase, methyltransferase, flavonol 7-, MT7, NOMT, OsOMT7

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.82 3-methylquercetin 7-O-methyltransferase

Engineering

Engineering on EC 2.1.1.82 - 3-methylquercetin 7-O-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D257G
-
the mutant methylates the 3-hydroxyl group of flavonols in addition to the 7-hydrdoxyl group
L101M
-
the mutant shows wild type reactivity with kaempferol and quercetin
additional information
-
generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene