2.1.1.74: methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing]

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing], go to the full flat file.

Reaction

Synonyms

EC 2.1.2.12, FDRTS, folate-dependent ribothymidyl synthase, folate-dependent tRNA methyltransferase, folate/FAD-dependent tRNA T54 methyltransferase, methylenetetrahydrofolate-transfer ribonucleate uracil 5-methyltransferase,, TRMFO, tRNA (m5U54)-methyltransferase, tRNA:m5U-54 MTase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.74 methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing]

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
11291	AA Sequence
8	Application
1	CAS Registry Number
5	Cloned(Commentary)
19	Cofactor
2	Crystallization (Commentary)
1	General Information
1	General Stability
11	KM Value [mM]
1	Localization
2	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
25	Organism
3	PDB ID
5	pH Optimum
17	Protein Variants
6	Purification (Commentary)
2	Reaction
2	Reaction Type
25	Reference
2	Specific Activity [micromol/min/mg]
45	Substrates and Products (Substrate)
33	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]

Cloned

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Cloned on EC 2.1.1.74 - methylenetetrahydrofolate-tRNA-(uracil54-C5)-methyltransferase [NAD(P)H-oxidizing]

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli as a His-tagged fusion protein

Bacillus subtilis

-

718903

expression in Escherichia coli

Thermotoga maritima

Q9WZJ3

706541

overexpression in Escherichia coli strain BL21(DE3)

Thermotoga maritima

-

701488

the encoded recombinant protein contains tightly bound flavin and is active in Escherichia coli mutant lacking m5U-54 in tRNAs and in vitro using T7 tRNA transcript as substrate

Bacillus subtilis

P39815

676202

untagged N-terminus and C-terminus (His)6-tagged TrmFO from Bacillus subtilis is expressed in Escherichia coli. The tag does not significantly alter the expression level, flavin content, activity and secondary structure of the protein

Bacillus subtilis

-

720967