Dam alters the Ca2+ regulation of Yop secretion but does not affect the temperature regulation of Yop/Ysc expression, in Dam-overproducing strains transcription of the lcrGV and yopN-tyeA operons is slightly upregulated, but LcrG is absent from lysates, while the amounts of YopN and TyeA are not changed, clpXP expression increases after Dam overproduction and the ClpP protease then degrades LcrG, thereby releasing a block in type III secretion
structural model of the type IC M.EcoR124I DNA methyltransferase, comprising the HsdS subunit, two HsdM subunits, the cofactor S-adenosyl-L-methionine and the substrate DNA molecule
the dimer stabilizes and enhances site-specific DNA-binding by the MTase, because the DNA-binding site may require an interfacial structure formed by both monomers, role of Type II MTases as defense systems against phage
the first Gua is recognized by K9, removal of which abrogates the first base-pair recognition, the flipped target Ade binds to the surface of EcoDam in the absence of S-adenosyl-L-methionine, which illustrates a possible intermediate in the base-flipping pathway, the orphaned Thy residue displays structural flexibility by adopting an extrahelical or intrahelical position where it is in contact to N120
confers antibiotic resistance in clinical isolates, important determinant of bacterial virulence, may be transmitted by horizontal gene transfer, potential drug target
epigenetic effects, in addition to genotoxic effects, need to be considered in chemical carcinogenesis initiated by r7,t8-dihydroxy-t9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene, since the inhibition of methylation may allow the expression of genes that promote tumor development
essential for the viability of the bacterium, overproduction of Dam in Aeromonas hydrophila SSU reduces the virulence of the pathogen, decreases the motility and T3SS-associated cytotoxicity, but increases both the cytotoxicity and hemolysis associated with Act, mice infected with the Dam-overproducing strain do not die over a tested period of 3 weeks, indicating bacterial attenuation as a result of Dam overproduction
methyltransferase activity associated with Dam is essential for attenuation of Aeromonas hydrophila virulence, immunization of mice with a native-Dam-overproducing strain at the same 50% lethal dose does not result in any lethality and provides protection to animals after subsequent challenge with a lethal dose of the control strain
upregulation of recombinational repair in dam mutants allows for the efficient repair of double-strand breaks whose formation is dependent on functional mismatch repair
essential for the viability of the bacterium, overproduction of Dam in Aeromonas hydrophila SSU reduces the virulence of the pathogen, decreases the motility and T3SS-associated cytotoxicity, but increases both the cytotoxicity and hemolysis associated with Act, mice infected with the Dam-overproducing strain do not die over a tested period of 3 weeks, indicating bacterial attenuation as a result of Dam overproduction
methyltransferase activity associated with Dam is essential for attenuation of Aeromonas hydrophila virulence, immunization of mice with a native-Dam-overproducing strain at the same 50% lethal dose does not result in any lethality and provides protection to animals after subsequent challenge with a lethal dose of the control strain
DNA-[adenine] MTase like gene is a pseudogene, no evidence of DNA-[adenine] methylation, DNA methylation may occur in a cell-cycle regulated manner, or as a developmental stage-specific event that does not include the red cell stage