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eriodictyol
?
-
best substrate, 131% relative activity
-
-
?
luteolin
?
-
88% relative activity
-
-
?
n-propyl gallate
?
best substrate
-
-
?
quercetin
?
-
71% relative activity
-
-
?
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid
S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid
S-adenosyl-L-methionine + 3,4-dihydroxy-5-methoxy-benzaldehyde
S-adenosyl-L-homocysteine + 4-hydroxy-3,5-dimethoxybenzaldehyde
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde
S-adenosyl-L-homocysteine + vanillin
S-adenosyl-L-methionine + 3-(3,4-dihydroxyphenyl)propanoic acid
S-adenosyl-L-homocysteine + 3-(4-hydroxy-3-methoxyphenyl)propanoic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
?
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + sinapaldehyde
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
?
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferyl aldehyde
?
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
S-adenosyl-L-methionine + 5-hydroxyferulic acid ethyl ester
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxy-trans-cinnamic acid
-
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
S-adenosyl-L-methionine + caffeic acid ethyl ester
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
?
S-adenosyl-L-methionine + caffeoyl alcohol
S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
S-adenosyl-L-methionine + caffeoyl alcohol
S-adenosyl-L-homocysteine + coniferyl alcohol
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
?
S-adenosyl-L-methionine + caffeoyl aldehyde
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
S-adenosyl-L-homocysteine + coniferyl aldehyde
S-adenosyl-L-methionine + caffeoyl aldehyde
S-adenosyl-L-homocysteine + ferulic aldehyde
-
-
-
-
?
S-adenosyl-L-methionine + catechol
S-adenosyl-L-homocysteine + 2-methoxyphenol
S-adenosyl-L-methionine + chlorogenic acid
?
-
-
-
-
?
S-adenosyl-L-methionine + daidzein
?
S-adenosyl-L-methionine + esculetin
?
-
-
-
-
?
S-adenosyl-L-methionine + genistein
?
-
poor substrate
-
-
?
S-adenosyl-L-methionine + homocatechol
S-adenosyl-L-homocysteine + 2-methoxy-4-methylphenol
-
-
-
-
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
S-adenosyl-L-methionine + protocatechuic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
S-adenosyl-L-methionine + protocatechuic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoic acid
-
-
-
-
?
S-adenosyl-L-methionine + protocatechuic aldehyde
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde
S-adenosyl-L-methionine + pyrogallol
?
-
poor substrate
-
-
?
S-adenosyl-L-methionine + quercetin
?
-
-
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + isorhamnetin
-
-
-
-
?
S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
additional information
?
-
myricetin
?
-
75% relative activity
-
-
?
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid
S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid
-
-
-
-
?
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid
S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid
-
-
-
-
?
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid
S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid
-
key lignin biosynthetic enzyme. Down-regulated transgenic plants show substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased digestibility
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
Bambusa sp.
-
-
i.e. ferulate
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
i.e. ferulate
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
i.e. ferulate
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
the enzyme does not use caffeic acid as a substrate in the presence of 5-hydroxyconiferaldehyde
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
i.e. ferulate
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
i.e. ferulate
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
i.e. ferulate
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeic acid, involved in the biosynthesis of lignin
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
i.e. caffeic acid, involved in the biosynthesis of lignin
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
i.e. ferulate
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeic acid, involved in the biosynthesis of lignin
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeate, m-specific methylation
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
enzyme essentially controls the biosynthesis of syringyl lignin units
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeic acid, involved in the biosynthesis of lignin
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeate, m-specific methylation
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
enzyme essentially controls the biosynthesis of syringyl lignin units
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
i.e. caffeic acid, involved in the biosynthesis of lignin and of some flavonoids in plants
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
the enzyme is co-induced with other enzymes of the general phenylpropanoid pathway and is involved in the biosynthesis of lignin precursors
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
-
-
?
S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde
S-adenosyl-L-homocysteine + vanillin
3,4-dihydroxybenzaldehyde is not the preferred substrate
i.e. 4-hydroxy-3-methoxybenzaldehyde
-
?
S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde
S-adenosyl-L-homocysteine + vanillin
3,4-dihydroxy-benzaldehyde is not the preferred substrate
i.e. 4-hydroxy-3-methoxybenzaldehyde
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
exhibits the highest catalyzing efficiency towards 5-hydroxyconiferaldehyde
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + sinapaldehyde
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + sinapaldehyde
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + sinapaldehyde
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
Bambusa sp.
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
at 70% the rate of caffeic acid methylation
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
at 70% the rate of caffeic acid methylation
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
best substrate
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
m-specific methylation
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
m-specific methylation
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
S-adenosyl-L-homocysteine + sinapic acid
-
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
?
-
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
?
-
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
?
-
100% relative activity
-
-
?
S-adenosyl-L-methionine + caffeic acid
?
-
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
-
-
-
-
r
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
-
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
?
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
?
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
?
-
alternative pathway to monolignols involving methylation of caffeoyl aldehyde and/or caffeoyl alcohol
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
-
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
?
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
?
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
?
-
alternative pathway to monolignols involving methylation of caffeoyl aldehyde and/or caffeoyl alcohol
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
S-adenosyl-L-homocysteine + coniferyl aldehyde
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
S-adenosyl-L-homocysteine + coniferyl aldehyde
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
S-adenosyl-L-homocysteine + coniferyl aldehyde
exhibits the highest catalyzing efficiency towards caffeoyl aldehyde
-
-
?
S-adenosyl-L-methionine + catechol
S-adenosyl-L-homocysteine + 2-methoxyphenol
-
-
-
-
?
S-adenosyl-L-methionine + catechol
S-adenosyl-L-homocysteine + 2-methoxyphenol
-
poor substrate
-
-
?
S-adenosyl-L-methionine + catechol
S-adenosyl-L-homocysteine + 2-methoxyphenol
-
isozyme II and III, no substrate for isozyme I
-
-
?
S-adenosyl-L-methionine + daidzein
?
-
poor substrate
-
-
?
S-adenosyl-L-methionine + daidzein
?
-
poor substrate
-
-
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
-
-
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
-
-
-
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
-
-
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
-
-
?
S-adenosyl-L-methionine + protocatechuic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
-
-
-
-
?
S-adenosyl-L-methionine + protocatechuic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
-
poor substrate
-
-
?
S-adenosyl-L-methionine + protocatechuic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
-
poor substrate
-
-
?
S-adenosyl-L-methionine + protocatechuic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
-
poor substrate
-
-
?
S-adenosyl-L-methionine + protocatechuic acid
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
-
-
-
-
?
S-adenosyl-L-methionine + protocatechuic aldehyde
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde
-
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S-adenosyl-L-methionine + protocatechuic aldehyde
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde
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S-adenosyl-L-methionine + protocatechuic aldehyde
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde
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S-adenosyl-L-methionine + protocatechuic aldehyde
S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde
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S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
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S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
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enzyme additionally shows flavone 3'-O-methyltransferase activity, reaction of EC 2.1.1.42, and N-acetylserotonin methyltransferase activity, reaction of EC 2.1.1.4. The catalytic efficiency for N-acetylserotonin methyltransferase activity is 709fold lower than for caffeic acid methyltransferase. In vitro, N-acetylserotonin methyltransferase activity is dramatically decreased by the addition of caffeic acid in a dose-dependent manner, but the activity of caffeic acid methyltransferase is not altered by N-acetylserotonin
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enzyme additionally shows flavone 3'-O-methyltransferase activity, reaction of EC 2.1.1.42, and N-acetylserotonin methyltransferase activity, reaction of EC 2.1.1.4. The catalytic efficiency for N-acetylserotonin methyltransferase activity is 709fold lower than for caffeic acid methyltransferase. In vitro, N-acetylserotonin methyltransferase activity is dramatically decreased by the addition of caffeic acid in a dose-dependent manner, but the activity of caffeic acid methyltransferase is not altered by N-acetylserotonin
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methylation only in meta-position
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molecular docking of potential substrates shows that the binding energy decreases in the order: thiol ester substrates, free acid substrates, alcoholic andaldehyde substrates, suggesting the best binding for thiol ester substrates
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bifunctional enzyme, methylation of 3-hydroxy and 5-hydroxy positions on the aromatic ring
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no activity with 3-hydroxybenzaldehyde, 4-hydroxybenzaldehyde, vanillin and syringaldehyde
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in silico studies suggest that alcoholic and aldehydic substrates are preferred to those of caffeic, sinapic, and ferulic acid by both caffeic acid-O-methyltransferase, and caffeoyl-coenzyme A-O-methyltransferase, EC 2.1.1.104, with a marked preference for CoA ester substrates over free acids, aldehydes, and alcohols
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p- and m-methylation of protocatechuic aldehyde and acid, as well as esculetin
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no acitivity with p-coumaric acid flavanones, naringenin, homoeriodictyol, hesperetin flavonols, kaempferol, isorhametin flavones, apigenin and chrysoeriol
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methylation only in meta-position
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enzyme RsOMT1 converts 3,5-dimethoxy-4-hydroxycinnamic, caffeic and 3,4,5-trihydroxybenzoic acids to trimethoxycinnamic-, ferulic/isoferulic- and 3-methoxy-4,5-dihydroxybenzoic, or 4-methoxy-3,5-dihydroxybenzoic acids, respectively, when supplied with these substrates
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enzyme RsOMT1 converts 3,5-dimethoxy-4-hydroxycinnamic, caffeic and 3,4,5-trihydroxybenzoic acids to trimethoxycinnamic-, ferulic/isoferulic- and 3-methoxy-4,5-dihydroxybenzoic, or 4-methoxy-3,5-dihydroxybenzoic acids, respectively, when supplied with these substrates
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enzyme RsOMT3 only converts caffeic acid to ferulic acid. The enzyme shows considerable promiscuity with respect to various flavonoid substrates that it accepts
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enzyme RsOMT3 only converts caffeic acid to ferulic acid. The enzyme shows considerable promiscuity with respect to various flavonoid substrates that it accepts
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enzyme is involved in biosynthesis of lignin
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random bi-bi mechanism. The observed reactivity hierarchy among 4,5-dihydroxy-3-methoxycinnamyl (and 3,4-dihydroxycinnamyl) aldehyde, alcohol, and acid substrates arises from the ability of the aldehyde to stabilize the anionic intermediate that results from deprotonation of the 5-hydroxyl group by histidine-267. Sinapaldehyde is the preferential product, as demonstrated by its low Km for 5-hydroxyconiferaldehyde. Unlike its acid and alcohol substrates, the aldehydes exhibit product inhibition, due to nonproductive binding of the S-cis-form of the aldehydes inhibiting productive binding of the S-trans-form
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additional information
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random bi-bi mechanism. The observed reactivity hierarchy among 4,5-dihydroxy-3-methoxycinnamyl (and 3,4-dihydroxycinnamyl) aldehyde, alcohol, and acid substrates arises from the ability of the aldehyde to stabilize the anionic intermediate that results from deprotonation of the 5-hydroxyl group by histidine-267. Sinapaldehyde is the preferential product, as demonstrated by its low Km for 5-hydroxyconiferaldehyde. Unlike its acid and alcohol substrates, the aldehydes exhibit product inhibition, due to nonproductive binding of the S-cis-form of the aldehydes inhibiting productive binding of the S-trans-form
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the enzyme exhibits also a strong activity towards ester precursors including caffeoyl-CoA and 5-hydroxyferuloyl-CoA
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substrate specificity, overview
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substrate specificity, overview
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does not have significant activity with the typical COMT substrates, caffeoyl aldehyde, 5-OH-coniferaldehyde, 5-OH-ferulic acid or caffeic acid or with the proposed vanillin precursor 3,4-dihydroxybenzaldehyde, thus is no component of the vanillin biosynthetic pathway, also no activity with kaempferol or quercetin
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does not have significant activity with the typical COMT substrates, caffeoyl aldehyde, 5-OH-coniferaldehyde, 5-OH-ferulic acid or caffeic acid or with the proposed vanillin precursor 3,4-dihydroxybenzaldehyde, thus is no component of the vanillin biosynthetic pathway, also no activity with kaempferol or quercetin
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additional information
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does not have significant activity with the typical COMT substrates, caffeoyl aldehyde, 5-OH-coniferaldehyde, 5-OH-ferulic acid or caffeic acid or with the proposed vanillin precursor 3,4-dihydroxybenzaldehyde, thus is no component of the vanillin biosynthetic pathway, also no activity with kaempferol or quercetin
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enzyme is involved in biosynthesis of lignin
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