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2.1.1.68: caffeate O-methyltransferase

This is an abbreviated version!
For detailed information about caffeate O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.68

Reaction

S-adenosyl-L-methionine
+
3,4-dihydroxy-trans-cinnamate
=
S-adenosyl-L-homocysteine
 +
3-methoxy-4-hydroxy-trans-cinnamate

Synonyms

3,4-dihydroxybenzaldehyde-O-methyltransferase, AtCOMT1, AtOMT1, benzenoid/phenylpropanoid meta/para-O-methyltransferase, Bmr12, Bradi3g16530, brown midrib 12, caffeate 3-O-methyltransferase, caffeate methyltransferase, caffeate/5-hydroxyferulate 3/5-O-methyltransferase, caffeic acid 3-O-methyltransferase, caffeic acid O-methyl-transferase, caffeic acid O-methyltransferase, caffeic acid O-methyltransferase 1, caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase, caffeic O-methyl transferase, CCOMT, COMT, COMT-3D, COMT1, COMT2, COMT4, COMT6, DOMT, FGCOMT1, Lp OMT1, O-methyltransferase, O-methytransferase, OMT, OMT1, OMT3, OsCOMT1, RsOMT1, RsOMT3, S-adenosyl-L-methionine:caffeic acid-O-methyltransferase, Sb07g003860, Van OMT-2, Van OMT-3

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.68 caffeate O-methyltransferase

Cloned

Cloned on EC 2.1.1.68 - caffeate O-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA clone Ptomt1 in Escherichia coli
-
cloning from phage library, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in strain JM109
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells and in Nicotiana tabacum cultivar Wisconsin 38
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells and calli from Oryza sativa cultivar Dongjin
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expression as N-terminal polyhistidine-tagged fusion in Escherichia coli BL21-CodonPlus (DE3)-RIPL competent cells based on the expression vector pET-15bexpression
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli XL-1 Blue
-
expression in Escherichia coli. Transgenic Festuca arundinacea plants carrying either sense or antisense COMT gene constructs are obtained by microprojectile bombardment of single-genotype-derived embryogenic suspension cells. Two co-suppressed transgenic lines are down-regulated in their lignin biosynthesis. These down-regulated transgenic plants show substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased digestibility
-
gene OMT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene OMT3, DNA and amino acid sequence determination and analysis, phylogenetic analysis
maize COMT gene expressed in Arabidopsis thaliana plants
-
recombinantly expressed as a His-tagged fusion protein
-