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2.1.1.6: catechol O-methyltransferase

This is an abbreviated version!
For detailed information about catechol O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.6

Reaction

S-adenosyl-L-methionine
+
a catechol
=
S-adenosyl-L-homocysteine
 +
a guaiacol

Synonyms

catechol methyltransferase, catechol-O-methyl transferase, catechol-O-methyl transferase, membrane bound, catechol-O-methyl transferase, soluble, catechol-O-methyltransferase, catechol-O-transferase, catecholamine O-methyltransferase, COMT, COMT I, COMT II, COMT-mb, COMT-s, COMT1, COMT2, CTOMT1, L-COMT, MB-COMT, methyltransferase, catechol, S-COMT, SCOMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.6 catechol O-methyltransferase

Purification

Purification on EC 2.1.1.6 - catechol O-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
commercial preparation
-
GST 4B Sepharose resin column chromatography, Super Q-5PW column chromatography, and Superdex 75 gel filtration
GST Sepharose resin column chromatography, GSTrap column chromatography, Super Q-5PW column chromatography, and Superdex 75 gel filtration
immobilized metal ion affinity chromatography
-
microsomal
-
overview on purification of native and recombinant protein
purification of recombinant human soluble COMT using hydrophobic interaction chromatography, as the main isolation method, from an Escherichia coli culture broth
-
recombinant enzyme
-
recombinant membrane-bound isoform, by arginine-affinity chromatography. Optimized conditions for isolation and purification consist in loading of 4 mg of total protein onto the column previously equilibrated at 20 °C and recovery of enzyme in a purified fraction using 500 mM NaCl, 10 mM DTT and 0.5% (v/v) Triton X-100 in 10 mM Tris buffer (pH 7) with a total bioactivity recovery of 24% and 4.95fold purification
-
recombinant protein, by arginine-affinity chromatography. Loading of 4 mg of total protein lysate leads to recovery of a purified fraction with a total bioactivity recovery of 24% and a purification fold of 4.95
soluble and microsomal enzyme
-
soluble form
-
TALON metal affinity resin column chromatography, Superdex 75 gel filtration