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2.1.1.56: mRNA (guanine-N7)-methyltransferase

This is an abbreviated version!
For detailed information about mRNA (guanine-N7)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.56

Reaction

S-adenosyl-L-methionine
+
a 5'-(5'-triphosphoguanosine)-[mRNA]
=
S-adenosyl-L-homocysteine
 +
a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]

Synonyms

(guanine-N7)-methyltransferase, Abd1, alphavirus capping enzyme, cap methyltransferase, cap N7-MTase, cap N7MTase, cap-methyltransferase, DENV methyltransferase, DENV-4 NS5, Ecm1, guanine-7-methyltransferase, guanine-N-7 methyltransferase, guanine-N7 methyltransferase, guanosine-7-methyltransferase, messenger ribonucleate guanine 7-methyltransferase, messenger RNA guanine 7-methyltransferase, methyltransferase domain of Sudan ebolavirus L protein, methyltransferase, messenger ribonucleate guanine 7-, mRNA cap (guanine N-7) methyltransferase, mRNA cap (guanine N-7) MTase, mRNA cap (guanine-N7) methyltransferase, mRNA cap methyltransferase, mRNA guanosine cap by RNA guanine-7-methyltransferase, MTase+CTD, N-7-MTase, N7 methyltransferase, N7 MTase, N7-methyltransferase, N7-MTase, non-structural protein 1, nonstructural protein 14, nonstructural protein 5 methyltransferase, NS5, NS5MTase, nsp 14, nsP1 methyltransferase, nsP1 MTase, nsp14, RNA (guanine-N7) methyltransferase, RNA cap (guanine N-7) methyltransferase, RNA guanine-7 methyltransferase, RNA guanine-7-methyltransferase, RNA guanine-methyltransferase, RNA guanine-N7 methyltransferase, RNA N7-MTase, RNMT, TbCgm1, TbCmt1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.56 mRNA (guanine-N7)-methyltransferase

Purification

Purification on EC 2.1.1.56 - mRNA (guanine-N7)-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by immobilized-metal-affinity chromatography
-
by nickel-affinity chromatography
Co-NTA resin column chromatography
-
co-purifies with mRNA-guanylyltransferase
-
DNA-agarose affinity chromatography
enzyme and truncated mutants recombinant from Saccharomyces cerevisiae
-
from infected HeLa cells
-
His6-Smt3-Abd1 by nickel-nitrilotriacetic acid fast flow column chromatography, Abd1 free of the tag by gel filtration, to homogeneity
-
methyltransferase domain, and small subunit of mRNA capping enzyme, recombinant from E. coli
-
Ni-NTA column chromatography
Ni2+-NTA agarose column chromatography
-
recombinant Ecm1 mutant proteins, by nickel-agarose chromatography
recombinant from E. coli and recombinant from in vitro transcription and translation
-
recombinant from Spodoptera frugiperda cells
-
recombinant N-terminally His6-tagged enzyme as Smt3-fusion protein from Escherichia coli strain BL21(DE3), removal of His-Smt3 tag by specific protease Ulp1, purification by metal affinity chromatography and gel filtration
-
small and large subunit of mRNA capping enzyme , recombinant from E. coli
-
TbCmt1 protein purified from soluble extract by nickel-agarose, followed by phosphocellulose chromatography
-