Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.1.1.56: mRNA (guanine-N7)-methyltransferase

This is an abbreviated version!
For detailed information about mRNA (guanine-N7)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.56

Reaction

S-adenosyl-L-methionine
+
a 5'-(5'-triphosphoguanosine)-[mRNA]
=
S-adenosyl-L-homocysteine
+
a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA]

Synonyms

(guanine-N7)-methyltransferase, Abd1, alphavirus capping enzyme, cap methyltransferase, cap N7-MTase, cap N7MTase, cap-methyltransferase, DENV methyltransferase, DENV-4 NS5, Ecm1, guanine-7-methyltransferase, guanine-N-7 methyltransferase, guanine-N7 methyltransferase, guanosine-7-methyltransferase, messenger ribonucleate guanine 7-methyltransferase, messenger RNA guanine 7-methyltransferase, methyltransferase domain of Sudan ebolavirus L protein, methyltransferase, messenger ribonucleate guanine 7-, mRNA cap (guanine N-7) methyltransferase, mRNA cap (guanine N-7) MTase, mRNA cap (guanine-N7) methyltransferase, mRNA cap methyltransferase, mRNA guanosine cap by RNA guanine-7-methyltransferase, MTase+CTD, N-7-MTase, N7 methyltransferase, N7 MTase, N7-methyltransferase, N7-MTase, non-structural protein 1, nonstructural protein 14, nonstructural protein 5 methyltransferase, NS5, NS5MTase, nsp 14, nsP1 methyltransferase, nsP1 MTase, nsp14, RNA (guanine-N7) methyltransferase, RNA cap (guanine N-7) methyltransferase, RNA guanine-7 methyltransferase, RNA guanine-7-methyltransferase, RNA guanine-methyltransferase, RNA guanine-N7 methyltransferase, RNA N7-MTase, RNMT, TbCgm1, TbCmt1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.56 mRNA (guanine-N7)-methyltransferase

Crystallization

Crystallization on EC 2.1.1.56 - mRNA (guanine-N7)-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
6.5 mg/ml purified native or selenomethionine-labeled, or Hg-labeled enzyme in complex with S-adenosyl-L-methionine, S-adenosyl-L-homocysteine, or the cap guanylate plus S-adenosyl-L-homocysteine, in 20 mM Tris-HCl, pH 8.0, 220 mM NaCl, vapour diffusion against a well solution of 1.2 M sodium potassium tartrate, 50 mM bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, pH 6.0-6.2, and 20 mM DTT, X-ray diffraction structure determination and analysis at 2.0-2.7 A resolution, modeling
-
Ecm1-aza-S-adenosyl-L-methionine binary complex, by vapor diffusion, reveals that the inhibitor occupies the same site as S-adenosyl-L-methionine
Ecm1-sinefungin binary complex, by vapor diffusion, to 2.6 A resolution
structure-function relationships of the N7-MTase. The ExoN domain is closely involved in the activity of the N7-MTase. Two of the 12 critical residues essential for the N7-MTase are located at the N terminus of the core ExoN domain, reinforcing a role of the ExoN domain in the N7-MTase activity of nsp14. The other 10 critical residues are distributed throughout the N7-MTase domain but localized mainly in the S-adenosyl-L-methionine-binding pocket