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2.1.1.53: putrescine N-methyltransferase

This is an abbreviated version!
For detailed information about putrescine N-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.53

Reaction

S-adenosyl-L-methionine
+
putrescine
=
S-adenosyl-L-homocysteine
 +
N-methylputrescine

Synonyms

methyltransferase, putrescine, NtPMT, PMT, PMT1, PMT2, PMT3, PMT4, PMTI, PMTII, putrescine methyltransferase, putrescine N-methyltransferase, putrescine N-methyltransferase 1, putrescine N-methyltransferase I

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.53 putrescine N-methyltransferase

Cloned

Cloned on EC 2.1.1.53 - putrescine N-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AbPMT1 is expressed more strongly than ABPMT2 in the root
expressed in Arabidopsis thaliana roots or in the vascular tissues, including pericycles and endodermis, of the Hyoscyamus niger hairy root cultures
-
expressed in Escherichia coli
expressed in Escherichia coli M15[pREP4] cells
expressed in Escherichia coli Rosetta-gami (DE3), transformed with a pET-21d vector encoding pmt
-
expressed in Escherichia coli, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3
expressed in roots and leaves
-
expressed in Rosetta-gami (DE3) cells
expressed in xylem-tissues of roots, three genes isolated from Nicotiana sylvestris: NsPMT1, NsPMT2, NsPMT3, a highly conserved NsPMT 5'-flanking region is sufficient for tissue-specific and jasmonate-responsive expression of all three PMT genes
into pETat-32a(+) vector and expressed in Escherichia coli BL21trxB(DE3)
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells
pmt1 and pmt2 cloned into pET-21d vector, PMT1 expressed in Escherichia coli Rosetta-gami (DE3), PMT2 expressed in Escherichia coli BL21 (DE3) and Rosetta-gami (DE3), pmt1 yields active enzymes, while pmt2 expression results in insoluble protein
T1 progeny of transgenic Atropa belladonna, in which putrescine N-methyltransferase (EC 2.1.1.53) from Nicotiana tabacum (NtPMT) and hyoscyamine 6beta-hydroxylase (EC 1.14.11.14) from Hyoscyamus niger (HnH6H) are overexpressed, are established to investigate tropane alkaloids biosynthesis and distribution in ripe fruits, leaves, stems, primary roots and secondary roots under field conditions. Both NtPMT and HnH6H are detected at the transcriptional level in transgenic plants, whereas they are not detected in wild-type plants. Metabolically engineering Atropa belladonna based on overexpressing NtPMT and HnH6H shows a chemotype of rich scopolamine that greatly improves the commercial and pharmaceutical properties of Atropa belladonna
-
tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain