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2.1.1.45: thymidylate synthase

This is an abbreviated version!
For detailed information about thymidylate synthase, go to the full flat file.

Word Map on EC 2.1.1.45

Reaction

5,10-methylenetetrahydrofolate
+
dUMP
=
dihydrofolate
+
dTMP

Synonyms

BgDHFR-TS, bifunctional TS-DHFR, DFR-TS, DHFR-TS, DHFR–TS, dihydrofolate reductase-thymidylate synthase, dTMP synthase, HTS, human TS, LBRM_06_0830, methylenetetrahydrofolate:dUMP C-methyltransferase, More, Thy1, ThyA, thymidylate synthase, thymidylate synthase A, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase, ThyX, TMP synthetase, TMPS, TS, TS-DHFR, TSase, TYMS, Y110A7A.4

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.45 thymidylate synthase

Expression

Expression on EC 2.1.1.45 - thymidylate synthase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
decreased mRNA expression after treatment with sodium butyrate or suberoylanilide hydroxamic acid
-
DHFR activity in lysates of Escherichia coli expressing Tsf-TbDHFR-TS is about 6fold more active than those expressing His6-TbDHFR-TS. In addition, thymidylate synthase activity, which has proved elusive with the His6-protein, is detectable. Stabilization of the TS activity with dUMP, without affect on DHFR stability. No stabilisation observed with CH2THF and other pyrimidine nucleotides, including the uracil-containing ribonucleotides and deoxyribonucleotides, and the thymidine-containing deoxyribonucleotides, overview
the expression of thymidylate synthase is increased by about 6fold when embryos are treated with 0.001 mM 5-fluorouracil and there is a greater than 10fold increase in the enzyme protein level after treatment with 0.0004 mM ZD1694
-
there is an approximately 2fold inverse relationship between thymidylate synthase expression and response to 5-fluoruracil
-
thymidylate synthase antisense oligodeoxynucleotide (ODN) 491 decreases thymidylate protein levels in HeLa cells with a concomitant increase in sensitivity to thymidylate synthase-targeting chemotherapeutics. In MCF-7 cells, ODN491 treatment is less effective at reducing thymidylate synthase mRNA and does not reduce thymidylate synthase protein, nor does it enhance sensitivity to thymidylate synthase-targeting or other chemotherapeutics. Treatment with either ODN 83 or ODN 491 reduces 6-fluoro-dUMP binding in HeLa cells by 59% and 62%, respectively, compared with control
-
thymidylate synthase mRNA levels of large-cell lung carcinoma cells are comparable of those of squamous cell carcinoma cells, but significantly higher than those of adenocarcinoma cells
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