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2.1.1.35: tRNA (uracil54-C5)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (uracil54-C5)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.35

Reaction

S-adenosyl-L-methionine
+
uracil54 in tRNA
=
S-adenosyl-L-homocysteine
+
5-methyluracil54 in tRNA

Synonyms

5-methyluridine 54 tRNA methyltransferase, m5U 54 tRNA methyltransferase, m5U 54 tRNA MTase, m5U methyltransferase, m5U-methyltransferase, methyltransferase, transfer ribonucleate uracil 5-, PAB0719, PAB0719 enzyme, PabTrmU54, ribothymidyl synthase, rTase, RumA, RumA-type tRNA(uracil-54, C5)-methyltransferase, RUMT, S-adenosyl-L-methionine-dependent tRNA (uracil-54, C5)-methyltransferase, transfer RNA uracil 5-methyltransferase, transfer RNA uracil methylase, TrmA, TRMT2A, tRNA (uracil-54, C5)-methyltransferase, tRNA uracil 5-methyltransferase, tRNA(m5U54)methyltransferase, tRNA(uracil-5-)methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.35 tRNA (uracil54-C5)-methyltransferase

Engineering

Engineering on EC 2.1.1.35 - tRNA (uracil54-C5)-methyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C324A
-
level of total TrmA-associated peptide 41%, amount of m5U in total tRNA 4%, relative to the levels found in the wild-type
D299A
-
level of total TrmA-associated peptide 63%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type
E358K
-
level of total TrmA-associated peptide 59%, amount of m5U in total tRNA 9%, relative to the levels found in the wild-type
E358Q
mutation of the catalytic base Glu-358 to Gln arrests catalysis and allows isolation of the covalent protein-RNA complex for crystallization
F188A
-
level of total TrmA-associated peptide 84%, amount of m5U in total tRNA 22%, relative to the levels found in the wild-type
G220D
-
level of total TrmA-associated peptide 76%, amount of m5U in total tRNA 2%, relative to the levels found in the wild-type
G360D
-
level of total TrmA-associated peptide 53%, amount of m5U in total tRNA 1%, relative to the levels found in the wild-type
Q190A
-
level of total TrmA-associated peptide 77%, amount of m5U in total tRNA 14%, relative to the levels found in the wild-type
R302A
-
level of total TrmA-associated peptide 82%, amount of m5U in total tRNA 87%, relative to the levels found in the wild-type
W132C
-
level of total TrmA-associated peptide 102%, amount of m5U in total tRNA 34%, relative to the levels found in the wild-type
W202C
-
level of total TrmA-associated peptide 1%, amount of m5U in total tRNA 15%, relative to the levels found in the wild-type
C521A
-
mutant without methyltransferase activity, putative nucleophilic Cys-521 may be involved in Cys-U54 bond formation
G428D
-
mutant without methyltransferase activity, G-428 is located at the presumed S-adenosyl-L-methionine binding site