2.1.1.326: N-acetyldemethylphosphinothricin P-methyltransferase

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For detailed information about N-acetyldemethylphosphinothricin P-methyltransferase, go to the full flat file.

Reaction

2 S-adenosyl-L-methionine +

Synonyms

BcpD, P-methylase, PhpK, SD_1168

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.326 N-acetyldemethylphosphinothricin P-methyltransferase

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Results	in table
1	AA Sequence
3	Cloned(Commentary)
4	Cofactor
3	General Information
2	Metals/Ions
1	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
10	Organism
4	Pathway
1	Purification (Commentary)
1	Reaction
9	Reference
14	Substrates and Products (Substrate)
2	Subunits
8	Synonyms
1	Systematic Name

Cloned

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Cloned on EC 2.1.1.326 - N-acetyldemethylphosphinothricin P-methyltransferase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Kitasatospora phosalacinea

-

738957

expression in Streptomyces lividans

Streptomyces hygroscopicus

-

738468

SD_1168 from Shewanella denitrificans is cloned into pET-30a(+)for protein overexpression. SD_1168 is found exclusively in inclusion bodies when overexpressed in either Escherichia coli or Streptomyces lividans. All efforts to express soluble recombinant protein are unsuccessful. Therefore SD_1168 is refolded using an established refolding procedure

Shewanella denitrificans

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737755